Crystal structure of a new type of NADPH-dependent quinone oxidoreductase (QOR2) from Escherichia coli SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Crystal structure of a new type of NADPH-dependent quinone oxidoreductase (QOR2) from Escherichia coli
Author(s)
Kim, In-Kwon; Yim, Hyung-Soon; Kim, Min-Kyu; Kim, Dong-Won; Kim, Young-Min; Cha, Sun-Shin; Kangl, Sa-Ouk
Publication Year
2008-05-29
Abstract
Escherichia coli QOR2 [NAD(P)H-dependent quinone oxidoreductase; a ytfG gene product], which catalyzes two-electron reduction of methyl-1,4-benzoquinone, is a new type of quinone-reducing enzyme with distinct primary sequence and oligomeric conformation from previously known quinone oxidoreductases. The crystal structures of native QOR2 and the QOR2-NADPH (nicotinamide adenine dinucleotide phosphate, reduced form) complex reveal that QOR2 consists of two domains (N-domain and C-domain) resembling those of NmrA, a negative transcriptional regulator that belongs to the short-chain dehydrogenase/reductase family. The N-domain, which adopts the Rossmann fold, provides a platform for NADPH binding, whereas the C-domain, which contains a hydrophobic pocket connected to the NADPH-binding site, appears to play important roles in substrate binding. Asn143 near the NADPH-binding site has been identified to be involved in substrate binding and catalysis from structural and mutational analyses. Moreover, compared with wild-type strain, the qor2-overexpressing strain shows growth retardation and remarkable decrease in several enzymes involved in carbon metabolism, suggesting that QOR2 could play some physiological roles in addition to quinone reduction. (C) 2008 Elsevier Ltd. All rights reserved.
ISSN
0022-2836
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4500
DOI
10.1016/j.jmb.2008.04.003
Bibliographic Citation
JOURNAL OF MOLECULAR BIOLOGY, v.379, no.2, pp.372 - 384, 2008
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
LIVER ALCOHOL-DEHYDROGENASE; THERMUS-THERMOPHILUS HB8; X-RAY DIFFRACTION; ZETA-CRYSTALLIN; DT-DIAPHORASE; 3-DIMENSIONAL STRUCTURE; NAD(P)H-QUINONE OXIDOREDUCTASE; CELLULAR PHOSPHOPROTEIN; 2-ELECTRON REDUCTION; NEGATIVE MODULATION
Keywords
QOR2; NAD(P)H-dependent quinone oxidoreductase; short-chain dehydrogenase/reductase family; transcriptional regulator; carbon metabolism
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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