New ShK-like peptide from the jellyfish Nemopilema nomurai has human potassium voltage-gated channel-blocking activity
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Title
- New ShK-like peptide from the jellyfish Nemopilema nomurai has human potassium voltage-gated channel-blocking activity
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Author(s)
- Ye-Ji Kim; Jo, Ye Jin; Seung Eun Lee; Jungeun Kim; Jae-Pil Choi; Nayoung Lee; Won, Hyokyoung; Dong Ho Woo; Yum, Seung Shic
- KIOST Author(s)
- Jo, Ye Jin(조예진); Lee, Na Young(이나영); Yum, Seung Shic(염승식)
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Alternative Author(s)
- 조예진; 이나영; 원효경; 염승식
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Publication Year
- 2023-11-08
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Abstract
- We have identified a new human voltage-gated potassium channel (hKv1.3) blocker, NnK-1 (CKDHHTYGVY10CKDWKSSGEC20KKNPKGMRHF30CRKTCGFC38), in the jellyfish Nemopilema nomurai, based on its genomic information. The gene sequence encoding NnK-1 contains 5,408 base pairs, with five introns and six exons. The coding sequence of the NnK-1 precursor is 894 nucleotides long and encodes 297 amino acids, containing five presumptive ShK-like peptides. An electrophysiological assay demonstrated that the chemically synthesized fifth peptide, NnK-1, is an effective hKv1.3 blocker. A multiple sequence alignment with cnidarian Shk-like peptides, which have Kv1.3-blocking activity, revealed that four residues (3Asp, 25Lys, 33Lys, and 34Thr) of NnK-1, together with six cysteine residues, are conserved. Therefore, we hypothesize that these four residues are crucial for the binding of the toxins to voltage-gated potassium channels.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/44831
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Bibliographic Citation
- 2023 The 19th International Conference on Toxicogenomics(ICT-2023), pp.148, 2023
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Publisher
- 대한 독성 유전·단백체학회
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Type
- Conference
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Language
- Korean
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