New ShK-like peptide from the jellyfish Nemopilema nomurai has human potassium voltage-gated channel-blocking activity
DC Field | Value | Language |
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dc.contributor.author | Ye-Ji Kim | - |
dc.contributor.author | Jo, Ye Jin | - |
dc.contributor.author | Seung Eun Lee | - |
dc.contributor.author | Jungeun Kim | - |
dc.contributor.author | Jae-Pil Choi | - |
dc.contributor.author | Nayoung Lee | - |
dc.contributor.author | Won, Hyokyoung | - |
dc.contributor.author | Dong Ho Woo | - |
dc.contributor.author | Yum, Seung Shic | - |
dc.date.accessioned | 2023-11-17T06:30:21Z | - |
dc.date.available | 2023-11-17T06:30:21Z | - |
dc.date.created | 2023-11-09 | - |
dc.date.issued | 2023-11-08 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/44831 | - |
dc.description.abstract | We have identified a new human voltage-gated potassium channel (hKv1.3) blocker, NnK-1 (CKDHHTYGVY10CKDWKSSGEC20KKNPKGMRHF30CRKTCGFC38), in the jellyfish Nemopilema nomurai, based on its genomic information. The gene sequence encoding NnK-1 contains 5,408 base pairs, with five introns and six exons. The coding sequence of the NnK-1 precursor is 894 nucleotides long and encodes 297 amino acids, containing five presumptive ShK-like peptides. An electrophysiological assay demonstrated that the chemically synthesized fifth peptide, NnK-1, is an effective hKv1.3 blocker. A multiple sequence alignment with cnidarian Shk-like peptides, which have Kv1.3-blocking activity, revealed that four residues (3Asp, 25Lys, 33Lys, and 34Thr) of NnK-1, together with six cysteine residues, are conserved. Therefore, we hypothesize that these four residues are crucial for the binding of the toxins to voltage-gated potassium channels. | - |
dc.description.uri | 2 | - |
dc.language | Korean | - |
dc.publisher | 대한 독성 유전·단백체학회 | - |
dc.relation.isPartOf | 2023 The 19th International Conference on Toxicogenomics Abstrack Book | - |
dc.title | New ShK-like peptide from the jellyfish Nemopilema nomurai has human potassium voltage-gated channel-blocking activity | - |
dc.type | Conference | - |
dc.citation.conferenceDate | 2023-11-07 | - |
dc.citation.conferencePlace | KO | - |
dc.citation.conferencePlace | ICC호텔, 대전 | - |
dc.citation.endPage | 148 | - |
dc.citation.startPage | 148 | - |
dc.citation.title | 2023 The 19th International Conference on Toxicogenomics(ICT-2023) | - |
dc.contributor.alternativeName | 조예진 | - |
dc.contributor.alternativeName | 이나영 | - |
dc.contributor.alternativeName | 원효경 | - |
dc.contributor.alternativeName | 염승식 | - |
dc.identifier.bibliographicCitation | 2023 The 19th International Conference on Toxicogenomics(ICT-2023), pp.148 | - |
dc.description.journalClass | 2 | - |