A cold-adapted epoxide hydrolase from a strict marine bacterium, Sphingophyxis alaskensis SCIE SCOPUS KCI

Cited 11 time in WEB OF SCIENCE Cited 11 time in Scopus
Title
A cold-adapted epoxide hydrolase from a strict marine bacterium, Sphingophyxis alaskensis
Author(s)
Kang, Ji-Hyun; Woo, Jung-Hee; Kang, Sung Gyun; Hwang, Young-Ok; Kim, Sang-Jin
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2008-08
Abstract
An open reading frame (ORF) encoding a putative epoxide hydrolase (EHase) was identified by analyzing the genome sequence of Sphingoplyxis alaskensis. The EHase gene (seh) was cloned and expressed in E. coli. To facilitate purification, the gene was fused in-frame to 6x histidine at the C-terminus. The recombinant EHase (rSEH) was highly soluble and could be purified to apparent homogeneity by one step of metal affinity chromatography. The purified SEH displayed hydrolyzing activities toward various epoxides such as styrene oxide, glycidyl phenyl ether, epoxyhexane, epoxybutane, epichlorohydrin, and epifluorohydrin. The optimum activity toward styrene oxide was observed at pH 6.5 and 35 degrees C. The purified SEH showed a cold-adapted property, displaying more than 40% of activity at low temperature of 10 degrees C compared with the optimum activity. Despite the catalytic efficiency, the purified SEH did not hydrolyze various epoxides enantioselectively. K-m and k(cat) of SEH toward (R)-styrene oxide were calculated as 4 +/- 0.3 mM and 7.42 s(-1), respectively, whereas K-m and k(cat) of SEH toward (S)-styrene oxide were 5.25 +/- 0.3 mM and 10.08 s(-1), respectively.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4477
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.8, pp.1445 - 1452, 2008
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
AGROBACTERIUM-RADIOBACTER AD1; HYPERTHERMOPHILIC ARCHAEON; CATALYTIC MECHANISM; SEQUENCE ALIGNMENT; ENCODING GENE; CLONING; EXPRESSION; TYROSINE; ENANTIOSELECTIVITY; PURIFICATION
Keywords
epoxide hydrolase; genome sequence; cold-adapted; marine microorganism
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Related Researcher
Research Interests

marine biotechnology,microbiology,해양생명공학,미생물학

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