A novel drying film culture method applying a natural phenomenon: Increased carotenoid production by Haematococcus sp
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- A novel drying film culture method applying a natural phenomenon: Increased carotenoid production by Haematococcus sp
- Ryu, Yong Kyun; Lee, Won Kyu; Choi, Yong Woon; Kim, Taihun; Lee, Yeon-Ji; Park, Areumi; Kim, Tae Ho; Oh, Chul Hong; Heo, Soo Jin; Kim, Ji Hyung; Jeon, Ga Eun; Kang, Do-Hyung
- KIOST Author(s)
- Ryu, Yong Kyun(유용균); Lee, Won Kyu(이원규); Choi, Yong Woon(최운용); Kim, Taihun(김태훈); Lee, Yeon-Ji(이연지); Park, Areumi(박아름이); Kim, Tae Ho(김태호); Oh, Chul Hong(오철홍); Heo, Soo Jin(허수진); Kang, Do Hyung(강도형)
- 유용균; 이원규; 최운용; 김태훈; 이연지; 박아름이; 김태호; 오철홍; 허수진; 강도형
- Low productivity and high cost remain major bottlenecks for the large-scale production of Haematococcus sp. This study explored biomass production and carotenoid accumulation in Haematococcus sp. (KCTC 12348BP) using drying film culture. The broth-cultured strain (3.2 × 106 cells/mL, 0.83 ± 0.02 mg/mL for a 21 d culture) was cultured under various conditions (different inoculum volumes and mist feeding intervals) in waterless agar plates at 28 ± 0.5 °C, under fluorescent light (12 h light–dark cycle) for 1 month. The maximum biomass obtained was 17.60 ± 0.72 g/m2, while the maximum astaxanthin concentration was 8.23 ± 1.13 mg/g in the culture using 1 mL inoculum and 3 d feeding interval. Drought stress in drying film culture effectively induced the accumulation of carotenoids from β-carotene, facilitating the production of canthaxanthin via the astaxanthin biosynthesis pathway. This cost-effective culture system can increase the biomass and carotenoid pigment production in Haematococcus sp.
- Bioresource Technology, v.390, 2023
- Elsevier BV
- Microalga; Haematococcus; Biomass; Carotenoid; Affordable culture system
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