Efficacy of White Spot Syndrome Virus Protein VP28-Expressing Chlorella vulgaris as an Oral Vaccine for Shrimp
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Title
- Efficacy of White Spot Syndrome Virus Protein VP28-Expressing Chlorella vulgaris as an Oral Vaccine for Shrimp
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Author(s)
- Kim, Min-Jeong; Kim, Su-Hyun; Kim, Jong-Oh; Lee, Taek Kyun; Jang, In-Kwon; Choi, Tae-Jin
- KIOST Author(s)
- Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 이택견
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Publication Year
- 2023-10
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Abstract
- The white spot syndrome virus (WSSV) is the causative agent of white spot disease, which kills shrimp within a few days of infection. Although WSSV has a mortality rate of almost 100% and poses a serious threat to the shrimp farming industry, strategies for its prevention and treatment are extremely limited. In this study, we examined the efficacy of VP28, a recombinant WSSV protein expressed in Chlorella vulgaris (C. vulgaris), as an oral shrimp vaccine. When compared with the control group, in which WSSV had a cumulative mortality of 100%, shrimp treated with 5% VP28-expressing C. vulgaris in their feed only had a 20% cumulative mortality rate 12 days after the WSSV challenge. When compared with the nonvaccinated group, the transcription of anti-lipopolysaccharide factor, C-type lectin, and prophenoloxidase genes, which are involved in shrimp defense against WSSV infection, was upregulated 29.6 fold, 15.4 fold, and 11.5 fold, respectively. These findings highlight C. vulgaris as a potential host for industrial shrimp vaccine production.
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ISSN
- 1999-4915
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/44673
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DOI
- 10.3390/v15102010
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Bibliographic Citation
- Viruses, v.15, no.10, 2023
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Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
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Keywords
- WSSV; VP28; vaccine; Chlorella vulgaris; immunization
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Type
- Article
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Language
- English
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Document Type
- Article
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