Streptochlorin isolated from Streptomyces sp. induces apoptosis in human hepatocarcinoma cells through a reactive oxygen species-mediated mitochondrial pathway SCIE SCOPUS KCI

Cited 19 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Streptochlorin isolated from Streptomyces sp. induces apoptosis in human hepatocarcinoma cells through a reactive oxygen species-mediated mitochondrial pathway
Author(s)
Shin, D.Y.; Shin, H.J.; Kim, G.-Y.; Cheong, J.H.; Choi, I.-W.; Kim, S.-K.; Moon, S.-K.; Kng, H.S.; Choi, Y.H.
Publication Year
2008-11
Abstract
Streptochlorin is a small molecule isolated from marine Streptomyces sp. that is known to have antiangiogenic and anticancer properties. In this study, we examined the effects of this compound on reactive oxygen species (ROS) production and the association of these effects with apoptotic tumor cell death, using a human hepatocarcinoma Hep3B cell line. The results of this study demonstrated that streptochlorin mediates ROS production, and that this mediation is followed by a decrease in the mitochondrial membrane potential (MMP, ΔΨ m), activation of caspase-3, and downregulation of antiapoptotic Bcl-2 protein. The quenching of ROS generation by N-acetyl-L-cysteine administration, a scavenger of ROS, reversed the streptochlorin-induced apoptosis effects via inhibition of ROS production, MMP collapse, and the subsequent activation of caspase-3. These observations clearly indicate that ROS are involved in the early molecular events in the streptochlorin-induced apoptotic pathway. Taken together, our data imply that streptochlorin-induced ROS is a key mediator of MMP collapse, which leads to the caspase-3 activation, culminating in apoptosis. © The Korean Society for Microbiology and Biotechnology.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4428
DOI
10.4014/jmb.0800.124
Bibliographic Citation
Journal of Microbiology and Biotechnology, v.18, no.11, pp.1862 - 1867, 2008
Publisher
한국미생물·생명공학회
Keywords
Apoptosis; Hep3B; MMP; ROS; Streptochlorin
Type
Article
Language
English
Document Type
Article
Publisher
한국미생물·생명공학회
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