Streptochlorin isolated from Streptomyces sp. induces apoptosis in human hepatocarcinoma cells through a reactive oxygen species-mediated mitochondrial pathway SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Shin, D.Y. | - |
dc.contributor.author | Shin, H.J. | - |
dc.contributor.author | Kim, G.-Y. | - |
dc.contributor.author | Cheong, J.H. | - |
dc.contributor.author | Choi, I.-W. | - |
dc.contributor.author | Kim, S.-K. | - |
dc.contributor.author | Moon, S.-K. | - |
dc.contributor.author | Kng, H.S. | - |
dc.contributor.author | Choi, Y.H. | - |
dc.date.accessioned | 2020-04-20T10:40:14Z | - |
dc.date.available | 2020-04-20T10:40:14Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4428 | - |
dc.description.abstract | Streptochlorin is a small molecule isolated from marine Streptomyces sp. that is known to have antiangiogenic and anticancer properties. In this study, we examined the effects of this compound on reactive oxygen species (ROS) production and the association of these effects with apoptotic tumor cell death, using a human hepatocarcinoma Hep3B cell line. The results of this study demonstrated that streptochlorin mediates ROS production, and that this mediation is followed by a decrease in the mitochondrial membrane potential (MMP, ΔΨ m), activation of caspase-3, and downregulation of antiapoptotic Bcl-2 protein. The quenching of ROS generation by N-acetyl-L-cysteine administration, a scavenger of ROS, reversed the streptochlorin-induced apoptosis effects via inhibition of ROS production, MMP collapse, and the subsequent activation of caspase-3. These observations clearly indicate that ROS are involved in the early molecular events in the streptochlorin-induced apoptotic pathway. Taken together, our data imply that streptochlorin-induced ROS is a key mediator of MMP collapse, which leads to the caspase-3 activation, culminating in apoptosis. © The Korean Society for Microbiology and Biotechnology. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Streptochlorin isolated from Streptomyces sp. induces apoptosis in human hepatocarcinoma cells through a reactive oxygen species-mediated mitochondrial pathway | - |
dc.type | Article | - |
dc.citation.endPage | 1867 | - |
dc.citation.startPage | 1862 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 18 | - |
dc.citation.number | 11 | - |
dc.contributor.alternativeName | 신희재 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.18, no.11, pp.1862 - 1867 | - |
dc.identifier.doi | 10.4014/jmb.0800.124 | - |
dc.identifier.scopusid | 2-s2.0-64549097687 | - |
dc.identifier.wosid | 000261237000020 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001293175 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | Streptomyces | - |
dc.subject.keywordPlus | tumor cell line | - |
dc.subject.keywordPlus | acetylcysteine | - |
dc.subject.keywordPlus | caspase 3 | - |
dc.subject.keywordPlus | natural product | - |
dc.subject.keywordPlus | protein bcl 2 | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | streptochlorin | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | indole derivative | - |
dc.subject.keywordPlus | oxazole derivative | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | streptochlorin | - |
dc.subject.keywordPlus | antiangiogenic activity | - |
dc.subject.keywordPlus | antineoplastic activity | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell death | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | drug isolation | - |
dc.subject.keywordPlus | enzyme activation | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | human cell culture | - |
dc.subject.keywordPlus | liver cell carcinoma | - |
dc.subject.keywordPlus | mitochondrial membrane potential | - |
dc.subject.keywordPlus | mitochondrial respiration | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | Streptomyces | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | isolation and purification | - |
dc.subject.keywordPlus | liver cell carcinoma | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | mitochondrial membrane potential | - |
dc.subject.keywordPlus | mitochondrion | - |
dc.subject.keywordPlus | physiology | - |
dc.subject.keywordPlus | Streptomyces sp. | - |
dc.subject.keywordPlus | Apoptosis | - |
dc.subject.keywordPlus | Carcinoma, Hepatocellular | - |
dc.subject.keywordPlus | Cell Line, Tumor | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Indoles | - |
dc.subject.keywordPlus | Membrane Potential, Mitochondrial | - |
dc.subject.keywordPlus | Mitochondria | - |
dc.subject.keywordPlus | Oxazoles | - |
dc.subject.keywordPlus | Reactive Oxygen Species | - |
dc.subject.keywordPlus | Streptomyces | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Hep3B | - |
dc.subject.keywordAuthor | MMP | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Streptochlorin | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |