Monitoring nutrient impact on bacterial community composition during bioremediation of anoxic PAH-contaminated sediment SCIE SCOPUS KCI

Cited 13 time in WEB OF SCIENCE Cited 12 time in Scopus
Title
Monitoring nutrient impact on bacterial community composition during bioremediation of anoxic PAH-contaminated sediment
Author(s)
Kim, Myungsu; Bae, Seung Seob; Seol, Mijin; Lee, Jung-Hyun; Oh, Young-Sook
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2008-12
Abstract
Marine harbor sediments are frequently polluted with significant amount of polycyclic aromatic hydrocarbons (PAHs) some of which are naturally toxic, recalcitrant, mutagenic, and carcinogenic. To stimulate biodegradation of PAHs in PAH-contaminated sediments collected from near Gwangyang Bay, Korea, lactate was chosen as a supplementary carbonaceous substrate. Sediment packed into 600 ml air-tight jar was either under no treatment condition or lactate amended condition (1%, w/v). Microbial community composition was monitored by bacteria-specific and archaea-specific PCR-terminal restriction fragment length polymorphism (T-RFLP), in addition to measuring the residual PAH concentration. Results showed that lactate amendment enhanced biodegradation rate of PAHs in the sediment by 4 to 8 times, and caused a significant shift in archaebacterial community in terms of structure and diversity with time. Phylogenetic analysis of 23 archaeal clones with distinctive RFLP patterns among 288 archaeal clones indicated that majority of the archaeal members were closest to unculturable environmental rDNA clones from hydrocarbon-contaminated and/or methanogenesis-bearing sediments. Lactate amendment led to the enrichment of some clones that were most closely related to PAH-degrading Methanosarcina species. These results suggest a possible contribution of methanogenic community to PAH degradation and give us more insights on how to effectively remediate PAH-contaminated sediments.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4422
DOI
10.1007/s12275-008-0097-z
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.46, no.6, pp.615 - 623, 2008
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; MARINE HARBOR SEDIMENTS; ANAEROBIC DEGRADATION; ESTUARINE SEDIMENTS; ARCHAEAL DIVERSITY; INTRINSIC BIOREMEDIATION; MICROBIAL DIVERSITY; SOIL; REMEDIATION; ENVIRONMENTS
Keywords
anaerobic biodegradation; bacterial community; archaea; polycyclic aromatic hydrocarbon; anoxic sediment
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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