Applicability of fluorescein diacetate (FDA) and Calcein-AM to determine the viability of marine plankton SCOPUS KCI

Title
Applicability of fluorescein diacetate (FDA) and Calcein-AM to determine the viability of marine plankton
Author(s)
Baek, S.H.; Shin, K.
KIOST Author(s)
Baek, Seung Ho(백승호)Shin, Kyoungsoon(신경순)
Publication Year
2009
Abstract
Ballast water is widely recognized as a serious environmental problem due to the risk of introducing non-indigenous aquatic species. In this study we aimed to investigate measures which can minimize the transfer of aquatic organisms from ballast water. Securing more reliable technologies to determine the viability of aquatic organisms is an important initiative in ballast water management systems. To evaluate the viability of marine phytoplankton, we designed the staining methods of fluorescein diacetate (FDA) and Calcein-AM assay on each target species belonging to different groups, such as bacillariphyceae, dinophyceae, raphidophyceae, chrysophyceae, haptophyceae and chlorophyceae. The FDA method, which is based on measurements of cell esterase activity using a fluorimetric stain, was the best dye for determining live cells of almost all phytoplankton species, except several diatoms tested in this study. On the other hand, although fluorescence of Calcein-AM was very clear for a comparatively longer time, green fluorescence per cell volume was lacking in most of the tested species. According to the Flow CAM method, which is a continuous imaging technique designed to characterize particles, green fluorescence values of stained cells by FDA were significantly higher than those of Calcein-AM treatments and control, implying that the Flow CAM using FDA assay could be adapted as an important tool for distinguishing living cells from dead cells. Our results suggest that the FDA and Calcein-AM methods can be adapted for use on phytoplankton, though species-specific characters are greatly different from one organism to another.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4356
DOI
10.4217/OPR.2009.31.4.349
Bibliographic Citation
Ocean and Polar Research, v.31, no.4, pp.349 - 357, 2009
Subject
Bacillariophyta; Chlorophyceae; Chrysophyceae; Dinophyceae; Haptophyceae; Raphidophyceae
Keywords
Ballast water management systems; Calcein-AM; Flow CAM techniques; Fluorescein diacetate (FDA) assay; Phytoplankton cell viablility
Type
Article
Language
Korean
Document Type
Note
Related Researcher
Research Interests

Marine Ecology,Marine Environment,HABs,해양생태,해양환경,적조

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