Applicability of fluorescein diacetate (FDA) and Calcein-AM to determine the viability of marine plankton SCOPUS KCI

DC Field Value Language
dc.contributor.author Baek, S.H. -
dc.contributor.author Shin, K. -
dc.date.accessioned 2020-04-20T10:25:03Z -
dc.date.available 2020-04-20T10:25:03Z -
dc.date.created 2020-01-28 -
dc.date.issued 2009 -
dc.identifier.issn 1598-141X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4356 -
dc.description.abstract Ballast water is widely recognized as a serious environmental problem due to the risk of introducing non-indigenous aquatic species. In this study we aimed to investigate measures which can minimize the transfer of aquatic organisms from ballast water. Securing more reliable technologies to determine the viability of aquatic organisms is an important initiative in ballast water management systems. To evaluate the viability of marine phytoplankton, we designed the staining methods of fluorescein diacetate (FDA) and Calcein-AM assay on each target species belonging to different groups, such as bacillariphyceae, dinophyceae, raphidophyceae, chrysophyceae, haptophyceae and chlorophyceae. The FDA method, which is based on measurements of cell esterase activity using a fluorimetric stain, was the best dye for determining live cells of almost all phytoplankton species, except several diatoms tested in this study. On the other hand, although fluorescence of Calcein-AM was very clear for a comparatively longer time, green fluorescence per cell volume was lacking in most of the tested species. According to the Flow CAM method, which is a continuous imaging technique designed to characterize particles, green fluorescence values of stained cells by FDA were significantly higher than those of Calcein-AM treatments and control, implying that the Flow CAM using FDA assay could be adapted as an important tool for distinguishing living cells from dead cells. Our results suggest that the FDA and Calcein-AM methods can be adapted for use on phytoplankton, though species-specific characters are greatly different from one organism to another. -
dc.description.uri 3 -
dc.language Korean -
dc.subject Bacillariophyta -
dc.subject Chlorophyceae -
dc.subject Chrysophyceae -
dc.subject Dinophyceae -
dc.subject Haptophyceae -
dc.subject Raphidophyceae -
dc.title Applicability of fluorescein diacetate (FDA) and Calcein-AM to determine the viability of marine plankton -
dc.type Article -
dc.citation.endPage 357 -
dc.citation.startPage 349 -
dc.citation.title Ocean and Polar Research -
dc.citation.volume 31 -
dc.citation.number 4 -
dc.contributor.alternativeName 백승호 -
dc.contributor.alternativeName 신경순 -
dc.identifier.bibliographicCitation Ocean and Polar Research, v.31, no.4, pp.349 - 357 -
dc.identifier.doi 10.4217/OPR.2009.31.4.349 -
dc.identifier.scopusid 2-s2.0-77950423911 -
dc.type.docType Note -
dc.identifier.kciid ART001398473 -
dc.description.journalClass 3 -
dc.subject.keywordPlus Bacillariophyta -
dc.subject.keywordPlus Chlorophyceae -
dc.subject.keywordPlus Chrysophyceae -
dc.subject.keywordPlus Dinophyceae -
dc.subject.keywordPlus Haptophyceae -
dc.subject.keywordPlus Raphidophyceae -
dc.subject.keywordAuthor Ballast water management systems -
dc.subject.keywordAuthor Calcein-AM -
dc.subject.keywordAuthor Flow CAM techniques -
dc.subject.keywordAuthor Fluorescein diacetate (FDA) assay -
dc.subject.keywordAuthor Phytoplankton cell viablility -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
Appears in Collections:
South Sea Research Institute > Ballast Water Research Center > 1. Journal Articles
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