Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge
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Title
- Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge
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Author(s)
- Kwon, Soo Jae; Lee, Joungmin; Lee, Hyun Sook
- KIOST Author(s)
- Kwon, Soo Jae(권수재); Lee, Joungmin(이종민); Lee, Hyun Sook(이현숙)
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Alternative Author(s)
- 권수재; 이종민; 이현숙
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Publication Year
- 2022-12
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Abstract
- In this study, we report the phenotypic changes that occurred in the acetogenic bacterium Clostridium sp. AWRP as a result of an adaptive laboratory evolution (ALE) under the acetate challenge. Acetate-adapted strain 46 T-a displayed acetate tolerance to acetate up to 10 g L−1 and increased ethanol production in small-scale cultures. The adapted strain showed a higher cell density than AWRP even without exogenous acetate supplementation. 46 T-a was shown to have reduced gas consumption rate and metabolite production. It was intriguing to note that 46 T-a, unlike AWRP, continued to consume H2 at low CO2 levels. Genome sequencing revealed that the adapted strain harbored three point mutations in the genes encoding an electron-bifurcating hydrogenase (Hyt) crucial for autotrophic growth in CO2 + H2, in addition to one in the dnaK gene. Transcriptome analysis revealed that most genes involved in the CO2-fixation Wood-Ljungdahl pathway and auxiliary pathways for energy conservation (e.g., Rnf complex, Nfn, etc.) were significantly down-regulated in 46 T-a. Several metabolic pathways involved in dissimilation of nucleosides and carbohydrates were significantly up-regulated in 46 T-a, indicating that 46 T-a evolved to utilize organic substrates rather than CO2 + H2. Further investigation into degeneration in carbon fixation of the acetate-adapted strain will provide practical implications for CO2 + H2 fermentation using acetogenic bacteria for long-term continuous fermentation.
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ISSN
- 1664-302X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/43553
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DOI
- 10.3389/fmicb.2022.982442
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Bibliographic Citation
- Frontiers in Microbiology, v.13, 2022
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Publisher
- Frontiers Media S.A.
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Keywords
- acetogen; Clostridium sp. AWRP; adaptive laboratory evolution; transcriptome; acetate stress
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Type
- Article
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Language
- English
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