Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kwon, Soo Jae | - |
dc.contributor.author | Lee, Joungmin | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.date.accessioned | 2022-12-15T23:50:02Z | - |
dc.date.available | 2022-12-15T23:50:02Z | - |
dc.date.created | 2022-12-14 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/43553 | - |
dc.description.abstract | In this study, we report the phenotypic changes that occurred in the acetogenic bacterium Clostridium sp. AWRP as a result of an adaptive laboratory evolution (ALE) under the acetate challenge. Acetate-adapted strain 46 T-a displayed acetate tolerance to acetate up to 10 g L−1 and increased ethanol production in small-scale cultures. The adapted strain showed a higher cell density than AWRP even without exogenous acetate supplementation. 46 T-a was shown to have reduced gas consumption rate and metabolite production. It was intriguing to note that 46 T-a, unlike AWRP, continued to consume H2 at low CO2 levels. Genome sequencing revealed that the adapted strain harbored three point mutations in the genes encoding an electron-bifurcating hydrogenase (Hyt) crucial for autotrophic growth in CO2 + H2, in addition to one in the dnaK gene. Transcriptome analysis revealed that most genes involved in the CO2-fixation Wood-Ljungdahl pathway and auxiliary pathways for energy conservation (e.g., Rnf complex, Nfn, etc.) were significantly down-regulated in 46 T-a. Several metabolic pathways involved in dissimilation of nucleosides and carbohydrates were significantly up-regulated in 46 T-a, indicating that 46 T-a evolved to utilize organic substrates rather than CO2 + H2. Further investigation into degeneration in carbon fixation of the acetate-adapted strain will provide practical implications for CO2 + H2 fermentation using acetogenic bacteria for long-term continuous fermentation. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Frontiers Media S.A. | - |
dc.title | Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge | - |
dc.type | Article | - |
dc.citation.title | Frontiers in Microbiology | - |
dc.citation.volume | 13 | - |
dc.contributor.alternativeName | 권수재 | - |
dc.contributor.alternativeName | 이종민 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.identifier.bibliographicCitation | Frontiers in Microbiology, v.13 | - |
dc.identifier.doi | 10.3389/fmicb.2022.982442 | - |
dc.identifier.scopusid | 2-s2.0-85144996211 | - |
dc.identifier.wosid | 000899355400001 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | ACETYL-BETA-LYSINE | - |
dc.subject.keywordPlus | ENERGY-CONSERVATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ACID TOLERANCE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | SYNGAS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | LJUNGDAHLII | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordAuthor | acetogen | - |
dc.subject.keywordAuthor | Clostridium sp. AWRP | - |
dc.subject.keywordAuthor | adaptive laboratory evolution | - |
dc.subject.keywordAuthor | transcriptome | - |
dc.subject.keywordAuthor | acetate stress | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |