Alginate nanocapsules by water-in-oil emulsification and external gelation for drug delivery to fine dust stimulated keratinocytes SCIE SCOPUS

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Title
Alginate nanocapsules by water-in-oil emulsification and external gelation for drug delivery to fine dust stimulated keratinocytes
Author(s)
Fernando,Ilekuttige Priyan Shanura; Kirindage, Kirinde Gedara Isuru Sandanuwan; Jayasinghe, Arachchige Maheshika Kumari; Han, Eui Jeong; Lee, Chang-Moon; Heo, Soo Jin; Ahn, Ginnae
KIOST Author(s)
Heo, Soo Jin(허수진)
Alternative Author(s)
허수진
Publication Year
2022-10
Abstract
Methodologies for synthesizing drug-loaded alginate nanocapsules were optimized and indomethacin and phloroglucinol loading capacities were studied. Their biological effects were studied for ameliorating fine dust (FD) induced detrimental effects in keratinocytes. The 1 % alginate to oil phase ratio of 1:20 was the optimal parameter for water in oil emulsification. The oil phase was optimized to contain sunflower oil: span 80 ratios of 17:3. Nanocapsule drug encapsulation efficiencies were 36.91 ± 5.56 and 32.41 ± 4.05 % respectively for phloroglucinol (EG2P) and indomethacin (EG2I) while the loading capacities were 25.28 ± 3.36 and 23.15 ± 2.84 %. Dried nanocapsules indicated a 40–140 nm diameter range while their hydrodynamic diameter was 989.69 nm at pH 7.0. Nanocapsules swelling was pH-dependent and in releasing media of pH values 4.5, 7.4, and 8.5, the drug release indicated a complex mechanism of swelling, diffusion, and erosion while at pH 2.0 the drug release followed the non-Fickian release. EG2P and EG2I treatment dose-dependently lowered FD-induced intracellular ROS production, apoptosis and inflammatory responses mediated through the NF-κB pathway in FD stimulated HaCaT keratinocytes and reduced epidermal barrier degradation. Further research could investigate the use of this technique in formulating cosmeceuticals containing drug-loaded alginate nanocapsules for achieving controlled release. © 2022
ISSN
0141-8130
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/43114
DOI
10.1016/j.ijbiomac.2022.07.097
Bibliographic Citation
International Journal of Biological Macromolecules, v.218, pp.102 - 114, 2022
Publisher
Elsevier B.V.
Keywords
Alginate; Fine dust; Nanocapsules
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier B.V.
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