Characterization and its Potential Application of Two Esterases Derived from the Arctic Sediment Metagenome SCIE SCOPUS

Cited 80 time in WEB OF SCIENCE Cited 78 time in Scopus
Title
Characterization and its Potential Application of Two Esterases Derived from the Arctic Sediment Metagenome
Author(s)
Jeon, Jeong Ho; Kim, Jun-Tae; Kang, Sung Gyun; Lee, Jung-Hyun; Kim, Sang-Jin
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2009-06
Abstract
Two esterase genes (designated as estAT1 and estAT11, respectively) were cloned by activity-based screening of a fosmid library constructed with seashore sediment sample of the Arctic. The sequence analysis of the genes revealed that these esterase genes encoded proteins of 303 and 312 amino acids, respectively, and showed 40-50% identities to members of the hormone-sensitive lipase (HSL) family retaining a catalytic triad with a conserved GDSAG sequence and an oxyanion hole (HGGG). The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant proteins (rEstAT1 and rEstAT11) were purified to homogeneity. The purified EstAT1 and EstAT11 were active in a broad range of temperature from 20 to 40A degrees C with an optimum temperature at 30A degrees C. The activation energies of rEstAT1 and rEstAT11 to hydrolyze p-nitrophenyl esters of butyrate were determined to be 12.65 kcal/mol and 11.26 kcal/mol, respectively, indicating that they are cold-adapted esterases. The purified EstAT1 and EstAT11 could hydrolyze racemic ofloxacin esters, and further rEstAT11 hydrolyzed preferentially (S)-racemic ofloxacin butyl ester with an enantiomeric excess (ee(p)) value of 70.3%. This work represents an example that develops enzymes from the Arctic using metagenomic approach, potentially applicable to chiral resolution of heat-labile substrates.
ISSN
1436-2228
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4288
DOI
10.1007/s10126-008-9145-2
Bibliographic Citation
MARINE BIOTECHNOLOGY, v.11, no.3, pp.307 - 316, 2009
Publisher
SPRINGER
Subject
OPTICALLY-ACTIVE OFLOXACIN; SEQUENCE ALIGNMENT; LIPOLYTIC ENZYMES; MARINE BACTERIUM; BIOTECHNOLOGY; LIPASES; MICROORGANISMS; BIOCATALYSTS; HYDROLASE; LIBRARY
Keywords
Metagenome; Arctic sediment; Esterase; Enantioselectivity
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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