A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 13 time in WEB OF SCIENCE Cited 14 time in Scopus
Title
A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1
Author(s)
Lee, Hyun Sook; Cho, Yona; Kim, Yun-Jae; Lho, Tae-Ok; Cha, Sun-Shin; Lee, Jung-Hyun; Kang, Sung Gyun
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2009-11
Abstract
The TON_0002 gene, which is in close proximity to the DNA polymerase locus in Thermococcus onnurineus NA1, has been shown to encode an inorganic pyrophosphatase. Its genomic position and function suggest a role for pyrophosphate hydrolysis during DNA polymerization. This is the first report of an inorganic pyrophosphatase belonging to the haloacid dehalogenase superfamily, in which unique residues in motif I and II have been replaced with Trp and Gly, respectively. The optimum pyrophosphatase activity of the recombinant enzyme occurred at pH 6, and it displayed an absolute dependence on divalent metal ions, among which Ni2+ was the most efficient. The site-specific mutation of the Gly residue in motif II to Ala or Ser residue exhibited only a slight change in the enzymatic activity and the K-m value.
ISSN
0378-1097
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4238
DOI
10.1111/j.1574-6968.2009.01766.x
Bibliographic Citation
FEMS MICROBIOLOGY LETTERS, v.300, no.1, pp.68 - 74, 2009
Publisher
OXFORD UNIV PRESS
Subject
COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; HYPERTHERMOPHILIC ARCHAEON; PHOSPHOSERINE PHOSPHATASE; CRYSTAL-STRUCTURE; FAMILY; SUPERFAMILY; DNA; REVEALS
Keywords
inorganic pyrophosphatase; haloacid dehalogenase superfamily; Thermococcus onnurineus NA1
Type
Article
Language
English
Document Type
Article
Publisher
OXFORD UNIV PRESS
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse