A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1
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Title
- A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1
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Author(s)
- Lee, Hyun Sook; Cho, Yona; Kim, Yun-Jae; Lho, Tae-Ok; Cha, Sun-Shin; Lee, Jung-Hyun; Kang, Sung Gyun
- KIOST Author(s)
- Lee, Hyun Sook(이현숙); Kim, Yun Jae(김윤재); Lee, Jung Hyun(이정현); Kang, Sung Gyun(강성균)
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Alternative Author(s)
- 이현숙; 김윤재; 차선신; 이정현; 강성균
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Publication Year
- 2009-11
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Abstract
- The TON_0002 gene, which is in close proximity to the DNA polymerase locus in Thermococcus onnurineus NA1, has been shown to encode an inorganic pyrophosphatase. Its genomic position and function suggest a role for pyrophosphate hydrolysis during DNA polymerization. This is the first report of an inorganic pyrophosphatase belonging to the haloacid dehalogenase superfamily, in which unique residues in motif I and II have been replaced with Trp and Gly, respectively. The optimum pyrophosphatase activity of the recombinant enzyme occurred at pH 6, and it displayed an absolute dependence on divalent metal ions, among which Ni2+ was the most efficient. The site-specific mutation of the Gly residue in motif II to Ala or Ser residue exhibited only a slight change in the enzymatic activity and the K-m value.
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ISSN
- 0378-1097
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4238
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DOI
- 10.1111/j.1574-6968.2009.01766.x
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Bibliographic Citation
- FEMS MICROBIOLOGY LETTERS, v.300, no.1, pp.68 - 74, 2009
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Publisher
- OXFORD UNIV PRESS
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Subject
- COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; HYPERTHERMOPHILIC ARCHAEON; PHOSPHOSERINE PHOSPHATASE; CRYSTAL-STRUCTURE; FAMILY; SUPERFAMILY; DNA; REVEALS
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Keywords
- inorganic pyrophosphatase; haloacid dehalogenase superfamily; Thermococcus onnurineus NA1
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Type
- Article
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Language
- English
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Document Type
- Article
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