Dieckol induces cell cycle arrest by down-regulating CDK2/cyclin E in response to p21/p53 activation in human tracheal fibroblasts
SCIE
SCOPUS
Cited 5 time in
WEB OF SCIENCE
Cited 7 time in
Scopus
-
Title
- Dieckol induces cell cycle arrest by down-regulating CDK2/cyclin E in response to p21/p53 activation in human tracheal fibroblasts
-
Author(s)
- Heo, Seong-Yeong; Jeong, Min-Seon; Lee, Hyoung Shin; Park, Won Sun; Choi, Il-Whan; Yi, Myunggi; Jung, Won-Kyo
- KIOST Author(s)
- Heo, Seong Yeong(허성영)
-
Alternative Author(s)
- 허성영
-
Publication Year
- 2022-01
-
Abstract
- The phlorotannin derivative dieckol isolated from Ecklonia cava has been shown to exhibit anti-inflammatory, anti-bacterial, anti-oxidative anti-adipogenic and anti-stenosis activity. However, the role of dieckol in cyclin-dependent kinase 2 (CDK2)/cyclin E signalling, which regulates fibrosis development, has not yet been determined. In this study, we report that dieckol-suppressed cell proliferation through the cell cycle arrest of Hs680.Tr human tracheal fibroblasts. Following consecutive purification, dieckol was identified as a potent bioactive compound. The results showed that dieckol had significant anti-proliferative activity against Hs680.Tr human tracheal fibroblastsWestern blotting analysis also found that dieckol dose-dependently induced the cell cycle arrest of Hs680.Tr fibroblasts in the G0/G1 phase, accompanied by the downregulation of CDK2 and cyclin E and the upregulation of p21 and p53. As attested by molecular docking study, the dieckol interacted with the core interface residues in transforming growth factor-beta receptor with high affinity. These findings suggest that dieckol from E. cava inhibits the cell proliferation of Hs680.Tr, potentially through p21- and p53-mediated G0/G1 cell cycle arrest.
-
ISSN
- 0263-6484
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/42240
-
DOI
- 10.1002/cbf.3675
-
Bibliographic Citation
- Cell Biochemistry and Function, v.40, no.1, pp.71 - 78, 2022
-
Publisher
- John Wiley & Sons Inc.
-
Keywords
- human tracheal fibroblasts; cell cycle arrest; cyclin E; CDK2 complex; Dieckol; Ecklonia cava
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.