Transcriptome dynamics in benzo[a]pyrene exposed Hydra SCIE SCOPUS KCI

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Transcriptome dynamics in benzo[a]pyrene exposed Hydra
Author(s)
Lee, Na Yun; Woo, Seon Ock; Lee, Na Young; Jo, Ye Jin; Yamindago, Ade; Yum, Seung Shic
KIOST Author(s)
Lee, Na Yun(이나윤)Woo, Seon Ock(우선옥)Lee, Na Young(이나영)Jo, Ye Jin(조예진)Yum, Seung Shic(염승식)
Alternative Author(s)
이나윤; 우선옥; 이나영; 조예진; 염승식
Publication Year
2022-07
Abstract
Backgrounds Benzo[a]pyrene (BaP) is a well-known ecotoxicant that induces a wide spectrum of toxic effects in organisms, including carcinogenicity, teratogenicity, genotoxicity, and immunotoxicity. Thus, re-evaluation of its acute toxic effects in gene expression at sublethal concentration in experimental animal is essential to understand and/or predict metabolic and physiological changes in an organism after exposure. Objectives To understand the changes in acute toxicity by exposure time, differential gene expression profiling of Hydra magnipapillata was performed by DNA microarray after exposure to BaP. Results The median lethal concentrations of the animals (LC50) were determined to be 78.5, 53.6, and 28.9 mg/L after exposure to BaP for 24, 48, and 72 h, respectively. Morphological responses of hydra polyps to 50 mg/L of BaP by exposure time were observed. The gene expression levels of molecular chaperones, antioxidative enzymes were altered at the early phase of the exposure. Transcription of the genes related to development and differentiation; apoptosis and necrosis were affected by the 4 h BaP exposure group. After 12 h exposure, developmental processes, immune responses, and ion transport in hydra polyp seemed to be affected, since the transcriptions of the genes that related to those biological processes were induced or repressed by BaP exposure. Neurotransmission might be suppressed, but tumorigenesis and carcinogenesis, the DNA repair process might be induced in the 24 h BaP-exposed hydra group. Finally, tumorigenesis and carcinogenesis, and the DNA repair process seemed to be induced after 48 h exposure. Conclusion We successfully demonstrated the dynamic response of Hydra to BaP by exposure time at transcription level. The results could extend our knowledge on acute toxic effect of BaP at sublethal conditions.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/42072
DOI
10.1007/s13273-021-00203-z
Bibliographic Citation
Molecular & Cellular Toxicology, v.18, no.3, pp.349 - 358, 2022
Publisher
대한독성 유전단백체 학회
Keywords
Polycyclic aromatic hydrocarbons; Toxicity; Differential gene expression; Cnidaria
Type
Article
Language
English
Document Type
Article; Early Access
Publisher
대한독성 유전단백체 학회
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