Identification of Ruditapes philippinarum and Meretrix lusoria larvae using single cell PCR analysis and microscopic observation SCOPUS KCI

Title
Identification of Ruditapes philippinarum and Meretrix lusoria larvae using single cell PCR analysis and microscopic observation
Author(s)
Jung, S.W.; Kim, C.-S.; Yoo, J.-W.; Kim, Y.-O.; Lee, J.H.; Hong, J.-S.
KIOST Author(s)
Jung, Seung Won(정승원)Kim, Young Ok(김영옥)
Publication Year
2010
Abstract
Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was 156±22 μm in length, 126±12 μm in height, 92±14 μm in width with a length: height ratio of 1.23. Meretrix lusoria was 202±44 μm in length, 161±35 μm in height, 96±38 μm in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4195
DOI
10.4217/OPR.2010.32.3.247
Bibliographic Citation
Ocean and Polar Research, v.32, no.3, pp.247 - 254, 2010
Subject
bivalve; body shape; body size; coastal water; DNA; experimental study; genetic analysis; identification method; larva; morphology; observational method; polymerase chain reaction; scanning electron microscopy; South Chungcheong; South Korea; Taean; Bivalvia; Meretrix lusoria; Veneroida; Venerupis (Ruditapes) philippinarum
Keywords
Bivalve larvae; Meretrix lusoria; Microscopic observation; Ruditapes philippinarum; Single cell PCR
Type
Article
Language
Korean
Document Type
Article
Related Researcher
Research Interests

Marine ecology,Plankton,Protists,해양생태,부유생물,원생생물

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