Identification of Ruditapes philippinarum and Meretrix lusoria larvae using single cell PCR analysis and microscopic observation SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Jung, S.W. | - |
dc.contributor.author | Kim, C.-S. | - |
dc.contributor.author | Yoo, J.-W. | - |
dc.contributor.author | Kim, Y.-O. | - |
dc.contributor.author | Lee, J.H. | - |
dc.contributor.author | Hong, J.-S. | - |
dc.date.accessioned | 2020-04-20T09:25:17Z | - |
dc.date.available | 2020-04-20T09:25:17Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1598-141X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4195 | - |
dc.description.abstract | Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was 156±22 μm in length, 126±12 μm in height, 92±14 μm in width with a length: height ratio of 1.23. Meretrix lusoria was 202±44 μm in length, 161±35 μm in height, 96±38 μm in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature. | - |
dc.description.uri | 3 | - |
dc.language | Korean | - |
dc.subject | bivalve | - |
dc.subject | body shape | - |
dc.subject | body size | - |
dc.subject | coastal water | - |
dc.subject | DNA | - |
dc.subject | experimental study | - |
dc.subject | genetic analysis | - |
dc.subject | identification method | - |
dc.subject | larva | - |
dc.subject | morphology | - |
dc.subject | observational method | - |
dc.subject | polymerase chain reaction | - |
dc.subject | scanning electron microscopy | - |
dc.subject | South Chungcheong | - |
dc.subject | South Korea | - |
dc.subject | Taean | - |
dc.subject | Bivalvia | - |
dc.subject | Meretrix lusoria | - |
dc.subject | Veneroida | - |
dc.subject | Venerupis (Ruditapes) philippinarum | - |
dc.title | Identification of Ruditapes philippinarum and Meretrix lusoria larvae using single cell PCR analysis and microscopic observation | - |
dc.type | Article | - |
dc.citation.endPage | 254 | - |
dc.citation.startPage | 247 | - |
dc.citation.title | Ocean and Polar Research | - |
dc.citation.volume | 32 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 정승원 | - |
dc.contributor.alternativeName | 김영옥 | - |
dc.identifier.bibliographicCitation | Ocean and Polar Research, v.32, no.3, pp.247 - 254 | - |
dc.identifier.doi | 10.4217/OPR.2010.32.3.247 | - |
dc.identifier.scopusid | 2-s2.0-78149272681 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001485690 | - |
dc.description.journalClass | 3 | - |
dc.subject.keywordPlus | bivalve | - |
dc.subject.keywordPlus | body shape | - |
dc.subject.keywordPlus | body size | - |
dc.subject.keywordPlus | coastal water | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | experimental study | - |
dc.subject.keywordPlus | genetic analysis | - |
dc.subject.keywordPlus | identification method | - |
dc.subject.keywordPlus | larva | - |
dc.subject.keywordPlus | morphology | - |
dc.subject.keywordPlus | observational method | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | scanning electron microscopy | - |
dc.subject.keywordPlus | South Chungcheong | - |
dc.subject.keywordPlus | South Korea | - |
dc.subject.keywordPlus | Taean | - |
dc.subject.keywordPlus | Bivalvia | - |
dc.subject.keywordPlus | Meretrix lusoria | - |
dc.subject.keywordPlus | Veneroida | - |
dc.subject.keywordPlus | Venerupis (Ruditapes) philippinarum | - |
dc.subject.keywordAuthor | Bivalve larvae | - |
dc.subject.keywordAuthor | Meretrix lusoria | - |
dc.subject.keywordAuthor | Microscopic observation | - |
dc.subject.keywordAuthor | Ruditapes philippinarum | - |
dc.subject.keywordAuthor | Single cell PCR | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |