Role of inorganic micronutrients on cell growth, photosynthetic pigments and biochemical enrichment of Arthrospira (Spirulina) maxima

Title
Role of inorganic micronutrients on cell growth, photosynthetic pigments and biochemical enrichment of Arthrospira (Spirulina) maxima
Author(s)
Lee, Won Kyu; Ryu, Yong Kyun; Choi, Woon Yong; Kim, Tae Ho; Kang, Do Hyung
KIOST Author(s)
Lee, Won Kyu(이원규)Ryu, Yong Kyun(유용균)Choi, Yong Woon(최운용)Kim, Tae Ho(김태호)Kang, Do Hyung(강도형)
Alternative Author(s)
이원규; 유용균; 최운용; 김태호; 강도형
Publication Year
2020-11-16
Abstract
Underground seawater sampled at Jeju Island, Korea (so called Jeju magma seawater, JMS) has high amounts of inorganic micronutrients compared with the concentrations in natural seawater of zinc (Z, 0.019 mg/L), iron (F, 0.015 mg/L), manganese (M, 0.008 mg/L), vanadium (V, 0.015 mg/L), selenium (S, 0.013 mg/L) and germanium (G, 0.002 mg/L). The micronutrients are required for growth and photosynthetic metabolism of marine algae. Limited information has been reported on the relation between the inorganic micronutrients of JMS and marine algae while some studies have been reported for land plant cultivation. The purpose of this study was to investigate the combination effect of inorganic micronutrients of JMS on cell growth and biochemical compositions of cyanobacterium, Spirulina maxima. Screening experiment of cell growth was conducted in 10 mL-flasks for 14 days. Optical density (OD) of 64 modified culture media containing cells was measured by spectrophotometric measurement (λ=664 nm) which are made by mixed 6 mineral reagents under different concentration regimes referred by JMS concentration. After then, selected eight combinations of the micronutrients were used for 20 L-scale cultivation to compare the biochemical differences and growth performance in scale-up culture. In 10 mL-scale cultivation for screening, the highest OD value was recorded in the medium containing mixed combination of M and V (M+V), followed M+Z+V and M+Z+F combinations. In scale-up cultivation, the final biomass and specific growth rate of S. maxima in the culture media with single Z and mixed M+Z combinations were significantly higher than others. Protein levels of cells were significantly high in mixed M+Z combination (p<0.05). Photosynthetic pigments of chlorophyll-a and C-phycocyanin of cells were also significantly increased in the media of single Z and mixed M+Z+V combinations, respectively (p<0.05). These results indicated that the M and Z of JMS have significant effects on cell growth and biochemical compositions of S. maxima.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41650
Bibliographic Citation
International Union of Microbiological Societies Congresses (IUMS 2020), 2020
Publisher
International Union of Microbiological Societies
Type
Conference
Language
English
Publisher
International Union of Microbiological Societies
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