Protective effects of enzymatic digest from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells SCIE SCOPUS

Cited 18 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Protective effects of enzymatic digest from Ecklonia cava against high glucose-induced oxidative stress in human umbilical vein endothelial cells
Author(s)
Lee, Seung-Hong; Heo, Soo-Jin; Hwang, Ji-Young; Han, Ji-Sook; Jeon, You-Jin
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2010-01-30
Abstract
BACKGROUND: Antioxidants can prevent pathological damage caused by hyperglycaemia-induced oxidative stress associated with diabetes. In the present study, we investigated whether the brown alga Ecklonia cava has protective effects against high glucose-induced oxidative stress in Human umbilical vein endothelial cells (HUVECs). For that purpose, we prepared an enzymatic digest from E. cava (ECC) by using the carbohydrase, Celluclast. RESULTS: High glucose treatment induced HUVECs cell death, but ECC, at a concentration of 10 or 100 mu g mL(-1), significantly inhibited the high glucose-induced cytotoxicity. Furthermore, treatment with ECC dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular generation of reactive oxygen species, and the nitric oxide level increased by high glucose. In addition, ECC treatment increased activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase in high-glucose pretreated HUVECs. High glucose levels induced the overexpression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear factor-kappa B proteins in HUVECs, but ECC treatment reduced the overexpression of these proteins. CONCLUSION: These results suggest that ECC is a potential therapeutic agent that will reduce the damage caused by hyperglycaemia-induced oxidative stress associated with diabetes. (C) 2009 Society of Chemical Industry
ISSN
0022-5142
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4144
DOI
10.1002/jsfa.3833
Bibliographic Citation
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.90, no.2, pp.349 - 356, 2010
Publisher
WILEY
Subject
NITRIC-OXIDE SYNTHASE; KAPPA-B ACTIVATION; BROWN ALGA; LIPID-PEROXIDATION; DIABETES-MELLITUS; LLC-PK1 CELLS; SUPEROXIDE; RATS; PEROXYNITRITE; INHIBITORS
Keywords
Ecklonia cava; enzymatic digest; high glucose; HUVECs; oxidative stress; protective effects
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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