Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17 SCOPUS KCI

Title
Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17
Author(s)
Nikapitiya, C.; Oh, C.; Lee, Y.; Lee, S.; Whang, I.; Lee, J.
KIOST Author(s)
Oh, Chulhong(오철홍)
Publication Year
2010-03
Abstract
An agar-degrading Agarivorans sp. AG17 strain was isolated from the red seaweed Grateloupia filicina collected from Jeju Island. A beta-agarase gene from Agarivorans sp. AG17 was cloned and designated as agrA. agrA has a 2,985 bp coding region encoding 995 amino acids and was classified into the glycoside hydrolase family (GHF)-50. Predicted molecular mass of the mature protein was 105 kDa. His-tagged agrA was overexpressed in Escherichia coli and purified as a fusion protein. The enzyme showed 158.8 unit/mg specific activity (optimum temperature at 65°C and pH 5.5 in acetate buffer) with unique biochemical properties (high thermal and pH stabilities). Enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose by degrading agar, and hydrolyzed neoagaro-oligosaccharides were biologically active. Hence the purified enzyme has potential for use in industrial applications such as the development of cosmetics and pharmaceuticals.
ISSN
2234-1749
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4124
DOI
10.5657/fas.2010.13.1.036
Bibliographic Citation
Fisheries and Aquatic Sciences, v.13, no.1, pp.36 - 48, 2010
Publisher
한국수산과학회
Keywords
Agarivorans sp; Beta agarase; Ghf-50; Neoagaro-oligosaccharides; Thermostable
Type
Article
Language
English
Document Type
Article
Publisher
한국수산과학회
Related Researcher
Research Interests

Recombinant protein,Marine microorganisms,Functional proteins,재조합단백질,해양미생물,기능성단백질

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