Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17 SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Nikapitiya, C. | - |
dc.contributor.author | Oh, C. | - |
dc.contributor.author | Lee, Y. | - |
dc.contributor.author | Lee, S. | - |
dc.contributor.author | Whang, I. | - |
dc.contributor.author | Lee, J. | - |
dc.date.accessioned | 2020-04-20T08:55:04Z | - |
dc.date.available | 2020-04-20T08:55:04Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 2234-1749 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4124 | - |
dc.description.abstract | An agar-degrading Agarivorans sp. AG17 strain was isolated from the red seaweed Grateloupia filicina collected from Jeju Island. A beta-agarase gene from Agarivorans sp. AG17 was cloned and designated as agrA. agrA has a 2,985 bp coding region encoding 995 amino acids and was classified into the glycoside hydrolase family (GHF)-50. Predicted molecular mass of the mature protein was 105 kDa. His-tagged agrA was overexpressed in Escherichia coli and purified as a fusion protein. The enzyme showed 158.8 unit/mg specific activity (optimum temperature at 65°C and pH 5.5 in acetate buffer) with unique biochemical properties (high thermal and pH stabilities). Enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose by degrading agar, and hydrolyzed neoagaro-oligosaccharides were biologically active. Hence the purified enzyme has potential for use in industrial applications such as the development of cosmetics and pharmaceuticals. | - |
dc.description.uri | 3 | - |
dc.language | English | - |
dc.publisher | 한국수산과학회 | - |
dc.title | Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17 | - |
dc.type | Article | - |
dc.citation.endPage | 48 | - |
dc.citation.startPage | 36 | - |
dc.citation.title | Fisheries and Aquatic Sciences | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.identifier.bibliographicCitation | Fisheries and Aquatic Sciences, v.13, no.1, pp.36 - 48 | - |
dc.identifier.doi | 10.5657/fas.2010.13.1.036 | - |
dc.identifier.scopusid | 2-s2.0-79953277907 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001433597 | - |
dc.description.journalClass | 3 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | Agarivorans | - |
dc.subject.keywordPlus | Bacteria (microorganisms) | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | Grateloupia filicina | - |
dc.subject.keywordAuthor | Agarivorans sp | - |
dc.subject.keywordAuthor | Beta agarase | - |
dc.subject.keywordAuthor | Ghf-50 | - |
dc.subject.keywordAuthor | Neoagaro-oligosaccharides | - |
dc.subject.keywordAuthor | Thermostable | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |