Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17 SCOPUS KCI

DC Field Value Language
dc.contributor.author Nikapitiya, C. -
dc.contributor.author Oh, C. -
dc.contributor.author Lee, Y. -
dc.contributor.author Lee, S. -
dc.contributor.author Whang, I. -
dc.contributor.author Lee, J. -
dc.date.accessioned 2020-04-20T08:55:04Z -
dc.date.available 2020-04-20T08:55:04Z -
dc.date.created 2020-01-28 -
dc.date.issued 2010-03 -
dc.identifier.issn 2234-1749 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4124 -
dc.description.abstract An agar-degrading Agarivorans sp. AG17 strain was isolated from the red seaweed Grateloupia filicina collected from Jeju Island. A beta-agarase gene from Agarivorans sp. AG17 was cloned and designated as agrA. agrA has a 2,985 bp coding region encoding 995 amino acids and was classified into the glycoside hydrolase family (GHF)-50. Predicted molecular mass of the mature protein was 105 kDa. His-tagged agrA was overexpressed in Escherichia coli and purified as a fusion protein. The enzyme showed 158.8 unit/mg specific activity (optimum temperature at 65°C and pH 5.5 in acetate buffer) with unique biochemical properties (high thermal and pH stabilities). Enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose by degrading agar, and hydrolyzed neoagaro-oligosaccharides were biologically active. Hence the purified enzyme has potential for use in industrial applications such as the development of cosmetics and pharmaceuticals. -
dc.description.uri 3 -
dc.language English -
dc.publisher 한국수산과학회 -
dc.title Characterization of a glycoside hydrolase family 50 thermostable β-agarase AgrA from marine bacteria agarivorans sp. AG17 -
dc.type Article -
dc.citation.endPage 48 -
dc.citation.startPage 36 -
dc.citation.title Fisheries and Aquatic Sciences -
dc.citation.volume 13 -
dc.citation.number 1 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation Fisheries and Aquatic Sciences, v.13, no.1, pp.36 - 48 -
dc.identifier.doi 10.5657/fas.2010.13.1.036 -
dc.identifier.scopusid 2-s2.0-79953277907 -
dc.type.docType Article -
dc.identifier.kciid ART001433597 -
dc.description.journalClass 3 -
dc.description.isOpenAccess N -
dc.subject.keywordPlus Agarivorans -
dc.subject.keywordPlus Bacteria (microorganisms) -
dc.subject.keywordPlus Escherichia coli -
dc.subject.keywordPlus Grateloupia filicina -
dc.subject.keywordAuthor Agarivorans sp -
dc.subject.keywordAuthor Beta agarase -
dc.subject.keywordAuthor Ghf-50 -
dc.subject.keywordAuthor Neoagaro-oligosaccharides -
dc.subject.keywordAuthor Thermostable -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
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