북태평양 연어의 계군 구분 및 유전자 확산 추정

Title
북태평양 연어의 계군 구분 및 유전자 확산 추정
Alternative Title
Genetic stock identification and gene flow estimation among the chum salmon populations in the North Pacific using the nucleotide variations in mtDNA, COIII-ND3-ND4L region
Author(s)
이윤호; 강거영; 정웅식; 김고은; 김충곤; 성기백; 강수경; Seeb, James; 김수암; Beerli, Peter
KIOST Author(s)
Lee, Youn Ho(이윤호)Kim, Choong Gon(김충곤)
Publication Year
2005-10-30
Abstract
Genetic stock identification has employed a number of different methods. In terms of accuracy, reproducibility and comparability, single nucleotide polymorphisms (SNPs) which use difference in DNA sequences are getting more acceptances. For the chum salmon, we identified 53 variable sites within the 744 nucleotide‐long region of mtDNA, COIII‐ND3‐ND4L, from the comparison of 186 individuals, 48 from Korea, 44 from Japan, 45 from Alaska, 29 from Canada and 20 from the United States. SNPs in these variable sites comprise 49 haplotypes, some of which are specific to a certain population. Based on these SNPs, population‐specific PCR primers were designed in a way that the most 3'end nucleotide becomes specific to each haplotype and that the second to the last nucleotide at the 3'end mismatches the conserved nucleotide at the site. PCR with these primers amplified DNA distinctively depending on the origin of samples. On the other hand, some haplotypes are common in different populations, indicating presence of individual migration among them. Parsimony network of individual genealogies corroborates such gene flow and shows close genetic relationship between Korea and Japan populations and among Canada, Alaska, and the United States populations. Major gene flow occurs asymmetrically from Alaska to Canada populations (16±6 individuals per generation), from the United States to Canada populations (5.7±1.9), from the North America to the Asia populations (1.4±0.2), and from Japan to Korea populations (10±3). In the last example of the gene flows, however, human‐mediated transplantation of stocks for hatchery operation should be considered in its interpretation.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41213
Bibliographic Citation
2005 NPAFC-PICES joint symposium, pp.63, 2005
Publisher
NPCFC-PICES
Type
Conference
Language
English
Publisher
NPCFC-PICES
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