Identification of differentially expressed genes for artificially hibernated olive flounder, Paralichthys olivaceus.

Abstract

In order to understand the physiological change in an artificially hibernated olive flounder, Paralichthys olivaceus, we first investigated the genes associated with the artificial hibernation. Change of the expressed genes in the brain tissue of the artificially hibernated olive flounder was examined using RNA-seq technology and DEGseq R package and then gene ontology (GO) functional enrichment analysis was performed. A total of 915 differentially expressed genes including 468 up-regulated and 447 down-regulated genes (P-value <0.001) were identified. The GO of the differentially expressed genes (DEGs) revealed 45 significantly enriched GO terms (indicating up and down regulated genes), most of which were associated with protein binding, synaptic transmission, and transcription factor activity. These genes are to be charactered further for function and involvement in the mechanisms of hibernation physiology. tissue of the artificially hibernated olive flounder was examined using RNA-seq technology and DEGseq R package and then gene ontology (GO) functional enrichment analysis was performed. A total of 915 differentially expressed genes including 468 up-regulated and 447 down-regulated genes (P-value <0.001) were identified. The GO of the differentially expressed genes (DEGs) revealed 45 significantly enriched GO terms (indicating up and down regulated genes), most of which were associated with protein binding, synaptic transmission, and transcription factor activity. These genes are to be charactered further for function and involvement in the mechanisms of hibernation physiology.