Genetic Identification of Korean Polychaetes collected from Chuja and Jeju island, using the DNA Barcode

Abstract

A total of 63 individuals belonging to 33 species in 8 order of polychaetes collected from Chuja and Jeju island were sequenced for a 658bp partial region of the mitochondrial cytochrome c oxidase subunit I(COI) gene, the typical molecular marker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful momarker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful mo