DNA barcode of spirotrich ciliates (Protozoa: Ciliophora) with emphasis on the high resolution among closely related species

Title
DNA barcode of spirotrich ciliates (Protozoa: Ciliophora) with emphasis on the high resolution among closely related species
Author(s)
정재호; 박미현; 최정민; 김영옥; 민기식
KIOST Author(s)
Choi, Jung Min(최정민)Kim, Young Ok(김영옥)
Alternative Author(s)
정재호; 최정민; 김영옥
Publication Year
2014-10-01
Abstract
The mitochondrial cytochrome c oxidase subunit I (COI) gene has been utilized for DNA barcoding to discriminate species. The CO1 barcode was also applied for ciliates and showed high resolution. However, previous primer pairs are not enough to uncover the class Spirotrichea of which one of species-rich groups in Ciliophora. Here we designed new primer pair for ciliate barcoding, especially for the class Spirotrichea. To design new primers, we sequenced mitochondrial genomes of two pseudokeronopsids by using next generation sequencing technique (HiSeq™ 2000). Based on the newly obtained CO1 gene fragments of the pseudokeronopsids, we designed new primer pair for spirotrichean ciliates and successfully sequenced CO1 barcode of 69 ciliate populations composed of 47 species. The CO1 barcode showed great potential to discriminate closely related species. We suggest reconsideration of Euplotes crassus as a distinct species, which was considered as a junior synonym of E. vannus, and found some candidates of cryptic species in Caudiholosticha sylvatica, Diophrys scutum, Euplotes crassus. Additionally, among the successfully sequenced 47 species, common species/genera were included and their barcodes will support to prove or reinforce biogeographical distribution pattern. to uncover the class Spirotrichea of which one of species-rich groups in Ciliophora. Here we designed new primer pair for ciliate barcoding, especially for the class Spirotrichea. To design new primers, we sequenced mitochondrial genomes of two pseudokeronopsids by using next generation sequencing technique (HiSeq™ 2000). Based on the newly obtained CO1 gene fragments of the pseudokeronopsids, we designed new primer pair for spirotrichean ciliates and successfully sequenced CO1 barcode of 69 ciliate populations composed of 47 species. The CO1 barcode showed great potential to discriminate closely related species. We suggest reconsideration of Euplotes crassus as a distinct species, which was considered as a junior synonym of E. vannus, and found some candidates of cryptic species in Caudiholosticha sylvatica, Diophrys scutum, Euplotes crassus. Additionally, among the successfully sequenced 47 species, common species/genera were included and their barcodes will support to prove or reinforce biogeographical distribution pattern.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40925
Bibliographic Citation
2nd Asian Marine Biology Symposium (AMBS), pp.104, 2014
Publisher
Organizing
Type
Conference
Language
English
Publisher
Organizing
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