DNA barcode of spirotrich ciliates (Protozoa: Ciliophora) with emphasis on the high resolution among closely related species

DC Field Value Language
dc.contributor.author 정재호 -
dc.contributor.author 박미현 -
dc.contributor.author 최정민 -
dc.contributor.author 김영옥 -
dc.contributor.author 민기식 -
dc.date.accessioned 2021-03-17T09:33:42Z -
dc.date.accessioned 2021-03-17T09:33:42Z -
dc.date.available 2021-03-17T09:33:42Z -
dc.date.available 2021-03-17T09:33:42Z -
dc.date.created 2020-02-11 -
dc.date.issued 2014-10-01 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/40925 -
dc.description.abstract The mitochondrial cytochrome c oxidase subunit I (COI) gene has been utilized for DNA barcoding to discriminate species. The CO1 barcode was also applied for ciliates and showed high resolution. However, previous primer pairs are not enough to uncover the class Spirotrichea of which one of species-rich groups in Ciliophora. Here we designed new primer pair for ciliate barcoding, especially for the class Spirotrichea. To design new primers, we sequenced mitochondrial genomes of two pseudokeronopsids by using next generation sequencing technique (HiSeq™ 2000). Based on the newly obtained CO1 gene fragments of the pseudokeronopsids, we designed new primer pair for spirotrichean ciliates and successfully sequenced CO1 barcode of 69 ciliate populations composed of 47 species. The CO1 barcode showed great potential to discriminate closely related species. We suggest reconsideration of Euplotes crassus as a distinct species, which was considered as a junior synonym of E. vannus, and found some candidates of cryptic species in Caudiholosticha sylvatica, Diophrys scutum, Euplotes crassus. Additionally, among the successfully sequenced 47 species, common species/genera were included and their barcodes will support to prove or reinforce biogeographical distribution pattern. to uncover the class Spirotrichea of which one of species-rich groups in Ciliophora. Here we designed new primer pair for ciliate barcoding, especially for the class Spirotrichea. To design new primers, we sequenced mitochondrial genomes of two pseudokeronopsids by using next generation sequencing technique (HiSeq™ 2000). Based on the newly obtained CO1 gene fragments of the pseudokeronopsids, we designed new primer pair for spirotrichean ciliates and successfully sequenced CO1 barcode of 69 ciliate populations composed of 47 species. The CO1 barcode showed great potential to discriminate closely related species. We suggest reconsideration of Euplotes crassus as a distinct species, which was considered as a junior synonym of E. vannus, and found some candidates of cryptic species in Caudiholosticha sylvatica, Diophrys scutum, Euplotes crassus. Additionally, among the successfully sequenced 47 species, common species/genera were included and their barcodes will support to prove or reinforce biogeographical distribution pattern. -
dc.description.uri 1 -
dc.language English -
dc.publisher Organizing -
dc.relation.isPartOf 2nd Asian Marine Biology Symposium (AMBS) -
dc.title DNA barcode of spirotrich ciliates (Protozoa: Ciliophora) with emphasis on the high resolution among closely related species -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 104 -
dc.citation.startPage 104 -
dc.citation.title 2nd Asian Marine Biology Symposium (AMBS) -
dc.contributor.alternativeName 정재호 -
dc.contributor.alternativeName 최정민 -
dc.contributor.alternativeName 김영옥 -
dc.identifier.bibliographicCitation 2nd Asian Marine Biology Symposium (AMBS), pp.104 -
dc.description.journalClass 1 -
Appears in Collections:
Marine Environmental & Climate Research Division > Marine Ecosystem Research Center > 2. Conference Papers
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