Avoiding and correcting chlorophyll fluorescence quenching by photoinhibition
- Avoiding and correcting chlorophyll fluorescence quenching by photoinhibition
- 안성민; 최동한; 양원석; 현명진; 이호원; 노재훈
- KIOST Author(s)
- Choi, Dong Han(최동한); Yang, Won Seok(양원석); Hyun, Myung Jin(현명진); Lee, Ho Won(이호원); Noh, Jae Hoon(노재훈)
- In-vivo fluorescence measurements are ideal for monitoring phytoplankton biomass.
However, as the PAR value increases under natural light conditions, phytoplankton protect
the photosynthetic system by non-photochemical quenching (NPQ) process. Fluorescence
quenching occurs during this process and the measured values using the fluorometer are
lower than the actual phytoplankton biomass. To overcome this problem, the dark
adaptation of the sample before measurement or thecorrection of the measurement results
is required. The calculation equation considering the PAR developed in this study showed a
good correction results. In addition, the effect on phytoplankton by diaphragm pump
included in the dark adaptation method was insignificant, and similar results were obtained
as the actual chlorophyll a concentration in the avoidance strategy of quenching by dark
adaptation. However, considering the time and space constraints of the field survey, the
method of calibration after observation using the underwater fluorescence sensor is more
appropriate. In particular, it can help to obtain large and coherent calibrated phytoplankton
biomass data monitored by autonomous platforms.
- 2019년 한국해양과학기술협의회 공동학술대회, 2019
- Files in This Item:
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.