Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury SCIE SCOPUS

Cited 35 time in WEB OF SCIENCE Cited 40 time in Scopus
Title
Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury
Author(s)
De Zoysa, Mahanama; Nikapitiya, Chamilani; Kim, Yucheol; Oh, Chulhong; Kang, Do-Hyung; Whang, Ilson; Kim, Se-Jae; Lee, Jae-Seong; Choi, Cheol Young; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)
Publication Year
2010-08
Abstract
Here, we report the identification and characterization of allograft inflammatory factor-1 (AIF-1) from disk abalone Haliotis discus discus that was denoted as AbAIF-1. The full-length cDNA of AbAIF-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kDa molecular mass. Analysis of AbAIF-1 sequence showed that it shares characteristic two EF hand Ca+2-binding motifs. Results from phylogenetic analysis further confirm that AbAIF-1 is a member of the AIF-1 family similar to invertebrate and vertebrate counterparts suggesting it has high evolutional conservation. Tissue-specific expression and transcriptional regulation of AbAIF-1 were analyzed after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes), viral hemorrhagic septicemia virus (VHSV) immune challenge and during tissue injury by quantitative real-time PCR. It is shown that the expression of AbAIF-1 mRNA was expressed ubiquitously in all selected tissues in constitutive manner showing the highest level in hemocytes. Upon bacteria and VHSV challenge, AbAIF-1 showed the significant up-regulation in hemocytes than gills. After the tissue injury in shell and mantle, AbAIF-1 and antioxidant selenium-dependant glutathione peroxidase (SeGPx) transcripts were significantly upregulated in abalone hemocytes. Taken together, these findings suggest that AIF-1 could response against the pathogenic challenge or tissue injury in abalone like mollusks. Also, AbAIF-1 may involve in wound healing and shell repair after the tissue injury of abalone. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4055
DOI
10.1016/j.fsi.2010.04.006
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.29, no.2, pp.319 - 326, 2010
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
GLUTATHIONE-PEROXIDASE; SEQUENCE ALIGNMENT; EXPRESSION; IDENTIFICATION; CYTOKINE; PROTEINS; VIRUS; AMYOTROPHIA; REJECTION; CHEMOKINE
Keywords
Abalone Haliotis discus discus; Allograft inflammatory factor-1; Invertebrate; Hemocytes; Tissue injury
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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