Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | De Zoysa, Mahanama | - |
dc.contributor.author | Nikapitiya, Chamilani | - |
dc.contributor.author | Kim, Yucheol | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | Kang, Do-Hyung | - |
dc.contributor.author | Whang, Ilson | - |
dc.contributor.author | Kim, Se-Jae | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.contributor.author | Choi, Cheol Young | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-20T08:40:09Z | - |
dc.date.available | 2020-04-20T08:40:09Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010-08 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4055 | - |
dc.description.abstract | Here, we report the identification and characterization of allograft inflammatory factor-1 (AIF-1) from disk abalone Haliotis discus discus that was denoted as AbAIF-1. The full-length cDNA of AbAIF-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kDa molecular mass. Analysis of AbAIF-1 sequence showed that it shares characteristic two EF hand Ca+2-binding motifs. Results from phylogenetic analysis further confirm that AbAIF-1 is a member of the AIF-1 family similar to invertebrate and vertebrate counterparts suggesting it has high evolutional conservation. Tissue-specific expression and transcriptional regulation of AbAIF-1 were analyzed after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes), viral hemorrhagic septicemia virus (VHSV) immune challenge and during tissue injury by quantitative real-time PCR. It is shown that the expression of AbAIF-1 mRNA was expressed ubiquitously in all selected tissues in constitutive manner showing the highest level in hemocytes. Upon bacteria and VHSV challenge, AbAIF-1 showed the significant up-regulation in hemocytes than gills. After the tissue injury in shell and mantle, AbAIF-1 and antioxidant selenium-dependant glutathione peroxidase (SeGPx) transcripts were significantly upregulated in abalone hemocytes. Taken together, these findings suggest that AIF-1 could response against the pathogenic challenge or tissue injury in abalone like mollusks. Also, AbAIF-1 may involve in wound healing and shell repair after the tissue injury of abalone. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.subject | GLUTATHIONE-PEROXIDASE | - |
dc.subject | SEQUENCE ALIGNMENT | - |
dc.subject | EXPRESSION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | CYTOKINE | - |
dc.subject | PROTEINS | - |
dc.subject | VIRUS | - |
dc.subject | AMYOTROPHIA | - |
dc.subject | REJECTION | - |
dc.subject | CHEMOKINE | - |
dc.title | Allograft inflammatory factor-1 in disk abalone (Haliotis discus discus): Molecular cloning, transcriptional regulation against immune challenge and tissue injury | - |
dc.type | Article | - |
dc.citation.endPage | 326 | - |
dc.citation.startPage | 319 | - |
dc.citation.title | FISH & SHELLFISH IMMUNOLOGY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | FISH & SHELLFISH IMMUNOLOGY, v.29, no.2, pp.319 - 326 | - |
dc.identifier.doi | 10.1016/j.fsi.2010.04.006 | - |
dc.identifier.scopusid | 2-s2.0-77954957023 | - |
dc.identifier.wosid | 000279388900019 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | AMYOTROPHIA | - |
dc.subject.keywordPlus | REJECTION | - |
dc.subject.keywordPlus | CHEMOKINE | - |
dc.subject.keywordPlus | GLUTATHIONE-PEROXIDASE | - |
dc.subject.keywordPlus | SEQUENCE ALIGNMENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Abalone Haliotis discus discus | - |
dc.subject.keywordAuthor | Allograft inflammatory factor-1 | - |
dc.subject.keywordAuthor | Invertebrate | - |
dc.subject.keywordAuthor | Hemocytes | - |
dc.subject.keywordAuthor | Tissue injury | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |