한국산 연어의 미토콘드리아 NADH Dehydrogenase Subunit 3 영역의 클로닝 및 DNA 염기서열 분석 KCI

Title
한국산 연어의 미토콘드리아 NADH Dehydrogenase Subunit 3 영역의 클로닝 및 DNA 염기서열 분석
Author(s)
최윤실; 이윤호; 진덕희
KIOST Author(s)
Lee, Youn Ho(이윤호)
Publication Year
2003-04
Abstract
Mitochondrial DNAs has been used frequently as genetic markers for the population genetic studies of salmonid fishes. Samples used in this experiment were chum salmons (Oncorhynchus keta) from Korea. We analyzed variation of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) among 4 individuals of the Korea population. Genomic DNA was extracted from the liver of the chum salmon samples. Then, the ND3 gene was amplified by polymerase chain reaction (PCR) including the 3' region of cytochrome oxidase III gene (COIII) and the 5` region of NADH dehydrogenase subunit 4L gene (ND4L). The size of the PCR product was 752 Up and the sequences showed some genetic variation among those four individuals. Genetic variations were observed in 7 sites as single nucleotide polymorphism (SNP). Within the open reading frame of the ND3 gene which encodes 116 amino acids, 5 nucleotide substitutions were found. Both transitional and transversional changes occurred more frequently with transitional changes. Comparison of these sequences with the others of a Japanese chum salmon in GenBank showed 5 sites of SNPs. This study provided the basic information of SNP in ND3 gene among Korean chum salmons and demonstrated the possible use of the SNP data as a genetic marker.
ISSN
0374-8111
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40524
Bibliographic Citation
한국수산과학회지, v.36, no.2, pp.94 - 99, 2003
Publisher
한국수산과학회
Keywords
Mitochondrial DNA; Chum salmon; COIII; ND3; ND4L; PCR; SNP
Type
Article
Publisher
한국수산과학회
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