Molecular phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida SCIE SCOPUS

Cited 73 time in WEB OF SCIENCE Cited 72 time in Scopus
Title
Molecular phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida
Author(s)
Lee, YH
KIOST Author(s)
Lee, Youn Ho(이윤호)
Publication Year
2003-08
Abstract
Sea urchins of the family Strongylocentrotidae have been important model systems in many fields of basic biology, yet knowledge of their evolutionary identities such as the phylogenetic relationships and divergence times remains limited. Here, I inferred molecular phylogenies of seven Strongylocentrotid species (Strongylocentrotus franciscanus, S. nudus, S. purpuratus, S. intermedius, S. droebachiensis, S. pallidus, and Hemicentrotus pulcherrimus) from the analyses of mitochondrial DNA sequences of 12SrDNA (349 nt), 12SrDNA-tRNA(gln) region (862 nt), and a combined sequence of cytochrome oxidase subunit I (COI, 1080 nt) and NADH dehydrogenase subunit I (NDI, 742 nt). The rate of sequence evolution and divergence times for each species were then estimated from the trees with reference to the time of separation between Strongylocentrotidae and Parechinidae, 35 to 50 MYA. The three trees agree well with each other, and the phylogeny is summarized by ((S. franciscanus, S. nudus), (H. pulcherrimus (S. purpuratus, S. intermedius (S. droebachiensis, S. pallidus)))). It is notable that the genus Strongylocentrotus consists of two distinct clades and that H. pulcherrimus branches off within Strongylocentrotus, implying assignment of a separate, monospecific genus to this species inappropriate. The rate of sequence evolution is calibrated to be 0.24%-0.34%/Myr in 12SrDNA, 0.25%-0.36%/Myr in 12SrDNA-tRNA(gln), and 0.65%-0.93%/Myr in COI-NDI combined sequences. S. purpuratus, in particular, shows the significantly higher rate of evolution in the 12SrDNA and 12SrDNA-tRNA(gln) regions compared to other species, suggesting careful use of its sequences in comparative studies. The two clades of Strongylocentrotidae seem to have split 13-19 MYA, and H. pulcherrimus branched off 7.2-14 MYA. In the former clade, S. franciscanus and S. nudus separated 5.7-8.1 MYA. In the latter clade, S. purpuratus, S. intermedius, and the clade of S. droebachiensis and S. pallidus diverged approximately 4.6-12 MYA, and the last two closest species separated 2.1-3.1 MYA.
ISSN
0737-4038
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40523
DOI
10.1093/molbev/msg125
Bibliographic Citation
MOLECULAR BIOLOGY AND EVOLUTION, v.20, no.8, pp.1211 - 1221, 2003
Publisher
OXFORD UNIV PRESS
Subject
MITOCHONDRIAL-DNA; POSITIVE SELECTION; NUCLEOTIDE-SEQUENCE; EVOLUTIONARY TREES; GENE ORGANIZATION; SPERM BINDIN; PROTEIN; CLOCK; ECHINOMETRA; POPULATIONS
Keywords
Strongylocentrotidae; sea urchin; phylogeny; evolutionary rate; divergence time; speciation
Type
Article
Language
English
Document Type
Article
Publisher
OXFORD UNIV PRESS
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