Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans SCIE SCOPUS

Cited 24 time in WEB OF SCIENCE Cited 26 time in Scopus
Title
Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans
Author(s)
Woo, Jung-Hee; Hwang, Young-Ok; Kang, Ji-Hyun; Lee, Hyun Sook; Kim, Sang-Jin; Kang, Sung Gyun
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2010-09
Abstract
Previously we reported that an epoxide hydrolase (EHase) from Novosphingobium aromaticivorans could preferentially hydrolyze (R)-styrene oxide. In this study, we demonstrate that the purified NEH could be also effective in chiral resolution of racemic epichlorohydrin (ECH). Particularly, the purified NEH showed excellent hydrolyzing activity toward ECH to complete the reaction at a short period of incubation time. Enantiopure (S)-ECH could be obtained with a high enantiopurity of more than 99.99% enantiomeric excess (ee) and yield of 20.7% (theoretical, 50%). The chiral resolution of the purified NEH toward ECH was not susceptible to substrate inhibition by 500 mM racemic ECH. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
ISSN
1389-1723
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4046
DOI
10.1016/j.jbiosc.2010.02.014
Bibliographic Citation
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.110, no.3, pp.295 - 297, 2010
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Subject
DIRECTED EVOLUTION; ERYTHROBACTER; CLONING
Keywords
Epichlorohydrin; Enantioselective; Epoxide hydrolase; Chiral resolution
Type
Article
Language
English
Document Type
Article
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Related Researcher
Research Interests

Microbial biotechnology,Marine enzymes,미생물 활용,효소 개발

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