Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans
SCIE
SCOPUS
Cited 24 time in
WEB OF SCIENCE
Cited 26 time in
Scopus
-
Title
- Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans
-
Author(s)
- Woo, Jung-Hee; Hwang, Young-Ok; Kang, Ji-Hyun; Lee, Hyun Sook; Kim, Sang-Jin; Kang, Sung Gyun
- KIOST Author(s)
- Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균)
-
Alternative Author(s)
- 우정희; 황영옥; 강지현; 이현숙; 김상진; 강성균
-
Publication Year
- 2010-09
-
Abstract
- Previously we reported that an epoxide hydrolase (EHase) from Novosphingobium aromaticivorans could preferentially hydrolyze (R)-styrene oxide. In this study, we demonstrate that the purified NEH could be also effective in chiral resolution of racemic epichlorohydrin (ECH). Particularly, the purified NEH showed excellent hydrolyzing activity toward ECH to complete the reaction at a short period of incubation time. Enantiopure (S)-ECH could be obtained with a high enantiopurity of more than 99.99% enantiomeric excess (ee) and yield of 20.7% (theoretical, 50%). The chiral resolution of the purified NEH toward ECH was not susceptible to substrate inhibition by 500 mM racemic ECH. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
-
ISSN
- 1389-1723
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4046
-
DOI
- 10.1016/j.jbiosc.2010.02.014
-
Bibliographic Citation
- JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.110, no.3, pp.295 - 297, 2010
-
Publisher
- SOC BIOSCIENCE BIOENGINEERING JAPAN
-
Subject
- DIRECTED EVOLUTION; ERYTHROBACTER; CLONING
-
Keywords
- Epichlorohydrin; Enantioselective; Epoxide hydrolase; Chiral resolution
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.