Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Woo, Jung-Hee | - |
dc.contributor.author | Hwang, Young-Ok | - |
dc.contributor.author | Kang, Ji-Hyun | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Kim, Sang-Jin | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.date.accessioned | 2020-04-20T08:40:05Z | - |
dc.date.available | 2020-04-20T08:40:05Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4046 | - |
dc.description.abstract | Previously we reported that an epoxide hydrolase (EHase) from Novosphingobium aromaticivorans could preferentially hydrolyze (R)-styrene oxide. In this study, we demonstrate that the purified NEH could be also effective in chiral resolution of racemic epichlorohydrin (ECH). Particularly, the purified NEH showed excellent hydrolyzing activity toward ECH to complete the reaction at a short period of incubation time. Enantiopure (S)-ECH could be obtained with a high enantiopurity of more than 99.99% enantiomeric excess (ee) and yield of 20.7% (theoretical, 50%). The chiral resolution of the purified NEH toward ECH was not susceptible to substrate inhibition by 500 mM racemic ECH. (C) 2010, The Society for Biotechnology, Japan. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | - |
dc.subject | DIRECTED EVOLUTION | - |
dc.subject | ERYTHROBACTER | - |
dc.subject | CLONING | - |
dc.title | Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans | - |
dc.type | Article | - |
dc.citation.endPage | 297 | - |
dc.citation.startPage | 295 | - |
dc.citation.title | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.citation.volume | 110 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 우정희 | - |
dc.contributor.alternativeName | 황영옥 | - |
dc.contributor.alternativeName | 강지현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.110, no.3, pp.295 - 297 | - |
dc.identifier.doi | 10.1016/j.jbiosc.2010.02.014 | - |
dc.identifier.scopusid | 2-s2.0-77955337162 | - |
dc.identifier.wosid | 000282927300006 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | DIRECTED EVOLUTION | - |
dc.subject.keywordPlus | ERYTHROBACTER | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordAuthor | Epichlorohydrin | - |
dc.subject.keywordAuthor | Enantioselective | - |
dc.subject.keywordAuthor | Epoxide hydrolase | - |
dc.subject.keywordAuthor | Chiral resolution | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |