Description of a New Planktonic Mixotrophic Dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp from the Coastal Waters off Western Korea: Morphology, Pigments, and Ribosomal DNA Gene Sequence SCIE SCOPUS

Cited 58 time in WEB OF SCIENCE Cited 67 time in Scopus
Title
Description of a New Planktonic Mixotrophic Dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp from the Coastal Waters off Western Korea: Morphology, Pigments, and Ribosomal DNA Gene Sequence
Author(s)
Kang, Nam Seon; Jeong, Hae Jin; Moestrup, Ojvind; Shin, Woongghi; Nam, Seung Won; Park, Jae Yeon; De Salas, Miguel F.; Kim, Ki Woo; Noh, Jae Hoon
KIOST Author(s)
Noh, Jae Hoon(노재훈)
Publication Year
2010-03
Abstract
The mixotrophic dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp. is described from living cells and from cells prepared by light, scanning electron, and transmission electron microscopy. In addition, sequences of the small subunit (SSU) and large subunit (LSU) rDNA and photosynthetic pigments are reported. The episome is conical, while the hyposome is hemispherical. Cells are covered with polygonal amphiesmal vesicles arranged in 16 rows and containing a very thin plate-like component. There is neither an apical groove nor apical line of narrow plates. Instead, there is a sulcal extension-like furrow. The cingulum is as wide as 0.2-0.3 x cell length and displaced by 0.2-0.3 x cell length. Cell length and width of live cells fed Amphidinium carterae were 8.4-19.3 and 6.1-16.0 mu m, respectively. Paragymnodinium shiwhaense does not have a nuclear envelope chamber nor a nuclear fibrous connective (NFC). Cells contain chloroplasts, nematocysts, trichocysts, and peduncle, though eyespots, pyrenoids, and pusules are absent. The main accessory pigment is peridinin. The sequence of the SSU rDNA of this dinoflagellate (GenBank AM408889) is 4% different from that of Gymnodinium aureolum, Lepidodinium viride, and Gymnodinium catenatum, the three closest species, while the LSU rDNA was 17-18% different from that of G. catenatum, Lepidodinium chlorophorum, and Gymnodinium nolleri. The phylogenetic trees show that this dinoflagellate belongs within the Gymnodinium sensu stricto clade. However, in contrast to Gymnodinium spp., cells lack nuclear envelope chambers, NFC, and an apical groove. Unlike Polykrikos spp., which have a taeniocyst-nematocyst complex, P. shiwhaense has nematocysts without taeniocysts. In addition, P. shiwhaense does not have ocelloids in contrast to Warnowia spp. and Nematodinium spp. Therefore, based on morphological and molecular analyses, we suggest that this taxon is a new species, also within a new genus.
ISSN
1066-5234
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40457
DOI
10.1111/j.1550-7408.2009.00462.x
Bibliographic Citation
JOURNAL OF EUKARYOTIC MICROBIOLOGY, v.57, no.2, pp.121 - 144, 2010
Publisher
WILEY
Subject
SP-NOV GYMNODINIALES; ELECTRON-MICROSCOPIC OBSERVATIONS; LSU RDNA SEQUENCES; FLAGELLAR APPARATUS; LAKE TOVEL; COMB. NOV; AMPHIDINIUM DINOPHYCEAE; PHYLOGENETIC ANALYSES; GREEN DINOFLAGELLATE; FEEDING-BEHAVIOR
Keywords
Dinoflagellate taxonomy; LSU; nematocyst; peduncle; SSU
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse