Molecular and biochemical characterizations of a novel arthropod endo-beta-1,3-glucanase from the Antarctic springtail, Cryptopygus antarcticus, horizontally acquired from bacteria SCIE SCOPUS

Cited 20 time in WEB OF SCIENCE Cited 21 time in Scopus
Title
Molecular and biochemical characterizations of a novel arthropod endo-beta-1,3-glucanase from the Antarctic springtail, Cryptopygus antarcticus, horizontally acquired from bacteria
Author(s)
Song, Jung Min; Nam, Kiwoong; Sun, Young-Uk; Kang, Mee Hye; Kim, Choong-Gon; Kwon, Suk-Tae; Lee, Jehee; Lee, Youn-Ho
KIOST Author(s)
Kim, Choong Gon(김충곤)Lee, Youn Ho(이윤호)
Publication Year
2010-04
Abstract
Collembolan species have been known to have beta,1,3-glucanase activity and yet the genes coding such enzymes have not been demonstrated. We report here a novel arthropod endo-beta-1,3-glucanase gene CaLam from the Antarctic springtail, Cryptopygus antarcticus. The open reading frame consists of 813 bp encoding 270 amino acids with a putative signal peptide and a typical motif of glycosyl hydrolase family 16 (GHF16), E-I-D-I-T-E. The recombinant protein expressed in E. coli shows the hydrolytic activity toward laminarin (K-m similar to 9.98 mg/mL) with an optimal temperature 50 degrees C and an optimal pH 6.0. CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products. Analyses of molecular phylogeny of CaLam and its protein structure reveal that CaLam is closely related with bacterial beta-1,3-glucanases more than with the eukaryotic homologues. Even so, the genomic structure of the CaLam gene consisting of six exons interspersed with approximately 57 to 63 bp introns confirms that it is endogenous in the genome of the Antarctic springtail. These results suggest that CaLam should have been transferred from bacteria to the lineage of the Collembolan species by horizontal gene transfer. (C) 2010 Elsevier Inc. All rights reserved.
ISSN
1096-4959
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40456
DOI
10.1016/j.cbpb.2010.01.003
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.155, no.4, pp.403 - 412, 2010
Publisher
ELSEVIER SCIENCE INC
Subject
BETA-1,3-GLUCAN RECOGNITION PROTEIN; HALIOTIS-DISCUS-HANNAI; CDNA CLONING; DIGESTIVE ENZYMES; BINDING-PROTEIN; MANDUCA-SEXTA; GENE-TRANSFER; PURIFICATION; ENDO-1,3-BETA-D-GLUCANASE; LIPOPOLYSACCHARIDE
Keywords
Endo-beta-1,3-glucanase; Laminarinase; Antarctic springtail; Cryptopygus antarcticus; Glycosyl hydrolase family 16 (GHF16); Horizontal gene transfer
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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