Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives SCIE SCOPUS

Cited 7 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives
Author(s)
Woo, Jung-Hee; Kwon, Tae-Hyung; Kim, Jun-Tae; Kim, Choong-Gon; Lee, Eun Yeol
KIOST Author(s)
Kim, Choong Gon(김충곤)
Publication Year
2013-04
Abstract
A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its derivatives, such as 2,3- and 4-chlorostyrene oxides, as a sole carbon source. Gordonia sp. H37 exhibited high enantioselective hydrolysis activity for 4-chlorostyrene oxide with an enantiomeric ratio of 27. Gordonia sp. H37 preferentially hydrolyzed the (R)-enantiomer of styrene oxide derivatives resulting in the preparation of a (S)-enantiomer with enantiomeric excess greater than 99.9 %. The enantioselective EHase activity was identified and characterized in various PAH-degrading bacteria, and whole cell Gordonia sp. H37 was employed as a biocatalyst for preparing enantiopure (S)-styrene oxide derivatives.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40431
DOI
10.1007/s10529-012-1114-1
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.35, no.4, pp.599 - 606, 2013
Publisher
SPRINGER
Subject
CHIRAL EPOXIDES; ERYTHROBACTER; CLONING
Keywords
Chlorostyrene oxides; Epoxide hydrolase; Gordonia sp.; Polycyclic aromatic hydrocarbon
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Molecular genetics,Zooplankton diversity,Marine healthcare,해양생물 분자유전,동물플랑크톤 다양성,해양헬스케어

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