Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives SCIE SCOPUS

DC Field Value Language
dc.contributor.author Woo, Jung-Hee -
dc.contributor.author Kwon, Tae-Hyung -
dc.contributor.author Kim, Jun-Tae -
dc.contributor.author Kim, Choong-Gon -
dc.contributor.author Lee, Eun Yeol -
dc.date.accessioned 2021-03-17T08:27:12Z -
dc.date.accessioned 2021-03-17T08:27:12Z -
dc.date.available 2021-03-17T08:27:12Z -
dc.date.available 2021-03-17T08:27:12Z -
dc.date.created 2020-02-04 -
dc.date.issued 2013-04 -
dc.identifier.issn 0141-5492 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/40431 -
dc.description.abstract A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its derivatives, such as 2,3- and 4-chlorostyrene oxides, as a sole carbon source. Gordonia sp. H37 exhibited high enantioselective hydrolysis activity for 4-chlorostyrene oxide with an enantiomeric ratio of 27. Gordonia sp. H37 preferentially hydrolyzed the (R)-enantiomer of styrene oxide derivatives resulting in the preparation of a (S)-enantiomer with enantiomeric excess greater than 99.9 %. The enantioselective EHase activity was identified and characterized in various PAH-degrading bacteria, and whole cell Gordonia sp. H37 was employed as a biocatalyst for preparing enantiopure (S)-styrene oxide derivatives. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject CHIRAL EPOXIDES -
dc.subject ERYTHROBACTER -
dc.subject CLONING -
dc.title Identification and characterization of epoxide hydrolase activity of polycyclic aromatic hydrocarbon-degrading bacteria for biocatalytic resolution of racemic styrene oxide and styrene oxide derivatives -
dc.type Article -
dc.citation.endPage 606 -
dc.citation.startPage 599 -
dc.citation.title BIOTECHNOLOGY LETTERS -
dc.citation.volume 35 -
dc.citation.number 4 -
dc.contributor.alternativeName 김충곤 -
dc.identifier.bibliographicCitation BIOTECHNOLOGY LETTERS, v.35, no.4, pp.599 - 606 -
dc.identifier.doi 10.1007/s10529-012-1114-1 -
dc.identifier.wosid 000316081800017 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus CHIRAL EPOXIDES -
dc.subject.keywordPlus ERYTHROBACTER -
dc.subject.keywordPlus CLONING -
dc.subject.keywordAuthor Chlorostyrene oxides -
dc.subject.keywordAuthor Epoxide hydrolase -
dc.subject.keywordAuthor Gordonia sp. -
dc.subject.keywordAuthor Polycyclic aromatic hydrocarbon -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
Appears in Collections:
Ocean Climate Solutions Research Division > Ocean Climate Response & Ecosystem Research Department > 1. Journal Articles
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