Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway
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Title
- Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway
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Author(s)
- Kim, Kil-Nam; Heo, Soo-Jin; Kang, Sung-Myung; Ahn, Ginnae; Jeon, You-Jin
- KIOST Author(s)
- Heo, Soo Jin(허수진)
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Alternative Author(s)
- 허수진
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Publication Year
- 2010-09
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Abstract
- Fucoxanthin, a natural biologically active substance isolated from Ishige okamurae, evidences antitumor activity in human leukemia cell HL-60 cells via the induction of apoptosis. However, the mechanism underlying fucoxanthin-induced apoptosis in HL-60 cells remains unclear. In this study, we focused on the effect of fucoxanthin induction on the accumulation of reactive oxygen species (ROS), and on the triggering of Bcl-xL signaling pathway in HL-60 cells. We determined that ROS are generated during fucoxanthin-induced cytotoxicity and apoptosis in HL-60 cells, and that N-acetylcysteine (NAC), a ROS scavenger, suppressed fucoxanthin-induced cytotoxicity and apoptosis. Moreover, fucoxanthin-induced the cleavage of caspases -3 and -7, and poly-ADP-ribose polymerase (PARP) and a decrease of Bcl-xL levels, whereas NAC pre-treatment significantly inhibited caspase-3, -7, and PARP cleavage and the reduction in Bcl-xL levels. In this study, it was demonstrated for the first time that fucoxanthin generated ROS and that the accumulation of ROS performed a crucial role in the fucoxanthin-induced Bcl-xL signaling pathway. (C) 2010 Elsevier Ltd. All rights reserved.
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ISSN
- 0887-2333
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4042
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DOI
- 10.1016/j.tiv.2010.05.023
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Bibliographic Citation
- TOXICOLOGY IN VITRO, v.24, no.6, pp.1648 - 1654, 2010
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Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
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Subject
- PROSTATE-CANCER CELLS; ACTIVATED PROTEIN-KINASE; OXIDATIVE STRESS; CYCLE ARREST; CARCINOMA-CELLS; DEATH; INDUCTION; CPP32; CAROTENOIDS; HOMOLOG
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Keywords
- Fucoxanthin; Reactive oxygen species; Apoptosis; Bcl-xL; Human leukemia HL-60 cells
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Type
- Article
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Language
- English
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Document Type
- Article
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