Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway SCIE SCOPUS

Cited 114 time in WEB OF SCIENCE Cited 129 time in Scopus
Title
Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway
Author(s)
Kim, Kil-Nam; Heo, Soo-Jin; Kang, Sung-Myung; Ahn, Ginnae; Jeon, You-Jin
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2010-09
Abstract
Fucoxanthin, a natural biologically active substance isolated from Ishige okamurae, evidences antitumor activity in human leukemia cell HL-60 cells via the induction of apoptosis. However, the mechanism underlying fucoxanthin-induced apoptosis in HL-60 cells remains unclear. In this study, we focused on the effect of fucoxanthin induction on the accumulation of reactive oxygen species (ROS), and on the triggering of Bcl-xL signaling pathway in HL-60 cells. We determined that ROS are generated during fucoxanthin-induced cytotoxicity and apoptosis in HL-60 cells, and that N-acetylcysteine (NAC), a ROS scavenger, suppressed fucoxanthin-induced cytotoxicity and apoptosis. Moreover, fucoxanthin-induced the cleavage of caspases -3 and -7, and poly-ADP-ribose polymerase (PARP) and a decrease of Bcl-xL levels, whereas NAC pre-treatment significantly inhibited caspase-3, -7, and PARP cleavage and the reduction in Bcl-xL levels. In this study, it was demonstrated for the first time that fucoxanthin generated ROS and that the accumulation of ROS performed a crucial role in the fucoxanthin-induced Bcl-xL signaling pathway. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
0887-2333
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4042
DOI
10.1016/j.tiv.2010.05.023
Bibliographic Citation
TOXICOLOGY IN VITRO, v.24, no.6, pp.1648 - 1654, 2010
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Subject
PROSTATE-CANCER CELLS; ACTIVATED PROTEIN-KINASE; OXIDATIVE STRESS; CYCLE ARREST; CARCINOMA-CELLS; DEATH; INDUCTION; CPP32; CAROTENOIDS; HOMOLOG
Keywords
Fucoxanthin; Reactive oxygen species; Apoptosis; Bcl-xL; Human leukemia HL-60 cells
Type
Article
Language
English
Document Type
Article
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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