Tumor suppressor QM-like gene from disk abalone (Haliotis discus discus): Molecular characterization and transcriptional analysis upon immune challenge SCIE SCOPUS

Cited 18 time in WEB OF SCIENCE Cited 15 time in Scopus
Title
Tumor suppressor QM-like gene from disk abalone (Haliotis discus discus): Molecular characterization and transcriptional analysis upon immune challenge
Author(s)
Oh, Chulhong; Zoysa, Mahanama De; Nikapitiya, Chamilani; Whang, Ilson; Kim, Yu Cheol; Kang, Do-Hyung; Heo, Soo-Jin; Choi, Young-Ung; Choi, Cheol Young; Lee, Jae-Seong; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)Heo, Soo Jin(허수진)
Publication Year
2010-09
Abstract
We describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor QM-like protein, AbQM, in the disk abalone nations discus discus. The full-length cDNA (765-bp) of AbQM was found to consist of a 654-bp ORF coding for a 218 amino acid protein of a 25 kDa molecular mass with a 10.2 isoelectric point. Analysis of AbQM sequence revealed the presence of characteristic motifs, including the ribosomal protein L10 signature, SH3-binding motif and two antibiotic binding sites. Phylogenetic analysis confirmed that AbQM is closely related to other mollusk QM proteins, and altogether they form a mollusk QM protein sub-family which displays evolutionary conservation from yeast to human Tissue-specific expression and transcriptional regulation of AbQM was analyzed by quantitative real-time PCR in response to bacterial (Vibrio alginolyticus and Vibrio parahemolyticus, Listeria monocrogenes) and viral (viral hemorrhagic septicemia virus, VHSV) challenge AbQM transcripts were found to be expressed ubiquitously in all examined tissues in a constitutive manner, as similar expression levels were detected in hemocytes, mantle, digestive tract and muscle Upon bacterial and VHSV challenge, AbQM showed significant up-regulation in gills, but not in hemocytes. Taken together, these findings suggest that AbQM in abalone-like mollusks can respond to and facilitate a defensive effect against pathogenic infection. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4040
DOI
10.1016/j.fsi.2010.05.007
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.29, no.3, pp.494 - 500, 2010
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
EXPRESSION ANALYSIS; SEQUENCE ALIGNMENT; PEARL OYSTER; C-JUN; HOMOLOG; PROTEIN; CLONING; STRESS; CELLS; CDNA
Keywords
Abalone Haliotis discus discus; Hemocytes; Invertebrate; QM gene; Tumor suppressor
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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