Formate-driven growth coupled with H-2 production SCIE SCOPUS

Cited 152 time in WEB OF SCIENCE Cited 156 time in Scopus
Title
Formate-driven growth coupled with H-2 production
Author(s)
Kim, Yun Jae; Lee, Hyun Sook; Kim, Eun Sook; Bae, Seung Seob; Lim, Jae Kyu; Matsumi, Rie; Lebedinsky, Alexander V.; Sokolova, Tatyana G.; Kozhevnikova, Darya A.; Cha, Sun-Shin; Kim, Sang-Jin; Kwon, Kae Kyoung; Imanaka, Tadayuki; Atomi, Haruyuki; Bonch-Osmolovskaya, Elizaveta A.; Lee, Jung-Hyun; Kang, Sung Gyun
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2010-09
Abstract
Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H-2 (with a change in Gibbs free energy of Delta G degrees = + 1.3 kJ mol(-1)) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ(1). The basis of the sustainable growth of the formate-users is explained by H-2 consumption by the methanogens, which lowers the H-2 partial pressure, thus making the pathway exergonic(2). However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H-2. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H-2-producing growth. The actual Delta G values for the formate metabolism are calculated to range between -8 and -20 kJ mol(-1) under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability.
ISSN
0028-0836
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4037
DOI
10.1038/nature09375
Bibliographic Citation
NATURE, v.467, no.7313, pp.352 - U137, 2010
Publisher
NATURE PUBLISHING GROUP
Type
Article
Language
English
Document Type
Article
Publisher
NATURE PUBLISHING GROUP
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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