Formate-driven growth coupled with H-2 production SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Yun Jae | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Kim, Eun Sook | - |
dc.contributor.author | Bae, Seung Seob | - |
dc.contributor.author | Lim, Jae Kyu | - |
dc.contributor.author | Matsumi, Rie | - |
dc.contributor.author | Lebedinsky, Alexander V. | - |
dc.contributor.author | Sokolova, Tatyana G. | - |
dc.contributor.author | Kozhevnikova, Darya A. | - |
dc.contributor.author | Cha, Sun-Shin | - |
dc.contributor.author | Kim, Sang-Jin | - |
dc.contributor.author | Kwon, Kae Kyoung | - |
dc.contributor.author | Imanaka, Tadayuki | - |
dc.contributor.author | Atomi, Haruyuki | - |
dc.contributor.author | Bonch-Osmolovskaya, Elizaveta A. | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.date.accessioned | 2020-04-20T08:25:37Z | - |
dc.date.available | 2020-04-20T08:25:37Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 0028-0836 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4037 | - |
dc.description.abstract | Although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and H-2 (with a change in Gibbs free energy of Delta G degrees = + 1.3 kJ mol(-1)) has not been considered energetic enough to support growth of microorganisms. Recently, experimental evidence for growth on formate was reported for syntrophic communities of Moorella sp. strain AMP and a hydrogen-consuming Methanothermobacter species and of Desulfovibrio sp. strain G11 and Methanobrevibacter arboriphilus strain AZ(1). The basis of the sustainable growth of the formate-users is explained by H-2 consumption by the methanogens, which lowers the H-2 partial pressure, thus making the pathway exergonic(2). However, it has not been shown that a single strain can grow on formate by catalysing its conversion to bicarbonate and H-2. Here we report that several hyperthermophilic archaea belonging to the Thermococcus genus are capable of formate-oxidizing, H-2-producing growth. The actual Delta G values for the formate metabolism are calculated to range between -8 and -20 kJ mol(-1) under the physiological conditions where Thermococcus onnurineus strain NA1 are grown. Furthermore, we detected ATP synthesis in the presence of formate as a sole energy source. Gene expression profiling and disruption identified the gene cluster encoding formate hydrogen lyase, cation/proton antiporter and formate transporter, which were responsible for the growth of T. onnurineus NA1 on formate. This work shows formate-driven growth by a single microorganism with protons as the electron acceptor, and reports the biochemical basis of this ability. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Formate-driven growth coupled with H-2 production | - |
dc.type | Article | - |
dc.citation.endPage | U137 | - |
dc.citation.startPage | 352 | - |
dc.citation.title | NATURE | - |
dc.citation.volume | 467 | - |
dc.citation.number | 7313 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 김은숙 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 차선신 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 권개경 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | NATURE, v.467, no.7313, pp.352 - U137 | - |
dc.identifier.doi | 10.1038/nature09375 | - |
dc.identifier.scopusid | 2-s2.0-77956936499 | - |
dc.identifier.wosid | 000281824900044 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | SEA HYDROTHERMAL VENT | - |
dc.subject.keywordPlus | HYPERTHERMOPHILIC ARCHAEON | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | METHANOBACTERIUM-FORMICICUM | - |
dc.subject.keywordPlus | METHANOCOCCUS-MARIPALUDIS | - |
dc.subject.keywordPlus | HYDROGENLYASE SYSTEM | - |
dc.subject.keywordPlus | OPERON | - |
dc.subject.keywordPlus | HYDROGENASE-3 | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PREDICTION | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |