Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1 SCIE SCOPUS KCI
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yeol Gyun | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Kim, Seung Il | - |
dc.contributor.author | Chung, Young-Ho | - |
dc.date.accessioned | 2020-04-20T08:25:16Z | - |
dc.date.available | 2020-04-20T08:25:16Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4001 | - |
dc.description.abstract | To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95 degrees C and pH 8.0 with a half-life (t(1/2)) of about 8 h. TNA1-Fbp had broad substrate specificities for fructose-1,6-bisphosphate and its analogues including fructose-l-phosphate, glucose-1-phosphate, and phosphoenolpyruvate. In addition, its enzyme activity was increased five-fold by addition of 1 mM Mg2+, while Li+ did not enhance enzymatic activity. TNA1-Fbp activity was inhibited by ATP, ADP, and phosphoenolpyruvate, but AMP up to 100 mM did not have any effect. TNA1-Fbp is currently defined as a class V fructose-1,6-bisphosphatase (FBPase) because it is very similar to FBPase of Thermococcus kodakaraensis KOD1 based on sequence homology. However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | COMPLETE GENOME SEQUENCE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | METABOLISM | - |
dc.subject | ACTIVATION | - |
dc.subject | ARCHAEA | - |
dc.title | Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1 | - |
dc.type | Article | - |
dc.citation.endPage | 807 | - |
dc.citation.startPage | 803 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 48 | - |
dc.citation.number | 6 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.48, no.6, pp.803 - 807 | - |
dc.identifier.doi | 10.1007/s12275-010-0377-2 | - |
dc.identifier.scopusid | 2-s2.0-78651341556 | - |
dc.identifier.wosid | 000285818600014 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001513212 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | COMPLETE GENOME SEQUENCE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | ARCHAEA | - |
dc.subject.keywordAuthor | T. onnurineus NA1 | - |
dc.subject.keywordAuthor | thermostability | - |
dc.subject.keywordAuthor | fructose-1,6-bisphosphatase | - |
dc.subject.keywordAuthor | hyperthermophilic Archaea | - |
dc.subject.keywordAuthor | fructose-1,6-bisphosphate | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |