Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1
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Title
- Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1
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Author(s)
- Lee, Yeol Gyun; Kang, Sung Gyun; Lee, Jung-Hyun; Kim, Seung Il; Chung, Young-Ho
- KIOST Author(s)
- Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 강성균; 이정현
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Publication Year
- 2010-12
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Abstract
- To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95 degrees C and pH 8.0 with a half-life (t(1/2)) of about 8 h. TNA1-Fbp had broad substrate specificities for fructose-1,6-bisphosphate and its analogues including fructose-l-phosphate, glucose-1-phosphate, and phosphoenolpyruvate. In addition, its enzyme activity was increased five-fold by addition of 1 mM Mg2+, while Li+ did not enhance enzymatic activity. TNA1-Fbp activity was inhibited by ATP, ADP, and phosphoenolpyruvate, but AMP up to 100 mM did not have any effect. TNA1-Fbp is currently defined as a class V fructose-1,6-bisphosphatase (FBPase) because it is very similar to FBPase of Thermococcus kodakaraensis KOD1 based on sequence homology. However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases.
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ISSN
- 1225-8873
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4001
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DOI
- 10.1007/s12275-010-0377-2
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Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.48, no.6, pp.803 - 807, 2010
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Publisher
- MICROBIOLOGICAL SOCIETY KOREA
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Subject
- COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; METABOLISM; ACTIVATION; ARCHAEA
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Keywords
- T. onnurineus NA1; thermostability; fructose-1,6-bisphosphatase; hyperthermophilic Archaea; fructose-1,6-bisphosphate
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Type
- Article
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Language
- English
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Document Type
- Article
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