Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1 SCIE SCOPUS KCI

Cited 3 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Characterization of Hyperthermostable Fructose-1,6-Bisphosphatase from Thermococcus onnurineus NA1
Author(s)
Lee, Yeol Gyun; Kang, Sung Gyun; Lee, Jung-Hyun; Kim, Seung Il; Chung, Young-Ho
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2010-12
Abstract
To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95 degrees C and pH 8.0 with a half-life (t(1/2)) of about 8 h. TNA1-Fbp had broad substrate specificities for fructose-1,6-bisphosphate and its analogues including fructose-l-phosphate, glucose-1-phosphate, and phosphoenolpyruvate. In addition, its enzyme activity was increased five-fold by addition of 1 mM Mg2+, while Li+ did not enhance enzymatic activity. TNA1-Fbp activity was inhibited by ATP, ADP, and phosphoenolpyruvate, but AMP up to 100 mM did not have any effect. TNA1-Fbp is currently defined as a class V fructose-1,6-bisphosphatase (FBPase) because it is very similar to FBPase of Thermococcus kodakaraensis KOD1 based on sequence homology. However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4001
DOI
10.1007/s12275-010-0377-2
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.48, no.6, pp.803 - 807, 2010
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; METABOLISM; ACTIVATION; ARCHAEA
Keywords
T. onnurineus NA1; thermostability; fructose-1,6-bisphosphatase; hyperthermophilic Archaea; fructose-1,6-bisphosphate
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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