The First Report to Evaluate Safety of Cyanobacterium Leptolyngbya sp. KIOST-1 for Use as a Food Ingredient: Oral Acute Toxicity and Genotoxicity Study SCIE SCOPUS KCI

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Title
The First Report to Evaluate Safety of Cyanobacterium Leptolyngbya sp. KIOST-1 for Use as a Food Ingredient: Oral Acute Toxicity and Genotoxicity Study
Alternative Title
The First Report to Evaluate Safety of Cyanobacterium Leptolyngbya sp. KIOST-1 for Use as a Food Ingredient: Oral Acute Toxicity and Genotoxicity Study
Author(s)
Lee, Youngdeuk; Kim, Taeho; Lee, Won Kyu; Ryu, Yong Kyun; Kim, Ji Hyung; Jeong, Younsik; Park, Areumi; Lee, Yeon Ji; Oh, Chulhong; Kang, Do-Hyung
KIOST Author(s)
Lee, Young Deuk(이영득)Kim, Tae Ho(김태호)Lee, Won Kyu(이원규)Ryu, Yong Kyun(유용균)Jeong, Youn Sik(정윤식)Park, Areumi(박아름이)Lee, Yeon Ji(이연지)Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)
Alternative Author(s)
이영득; 김태호; 이원규; 유용균; 정윤식; 박아름이; 이연지; 오철홍; 강도형
Publication Year
2021-02
Abstract
Leptolyngbya sp. KIOST-1 (LK1) is a newly isolated cyanobacterium that shows no obvious cytotoxicity and contains high protein content for both human and animal diets. However, only limited information is available on its toxic effects. The purpose of this study was to validate the safety of LK1 powder. Following Organisation for Economic Co-operation and Development (OECD) guidelines, a single-dose oral toxicity test in Sprague Dawley rats was performed. Genotoxicity was assessed using a bacterial reverse mutation test with Salmonella typhimurium (strains TA98, TA100, TA1535, and TA1537) and Escherichia coli WP2 uvrA, an in vitro mammalian chromosome aberration test using Chinese hamster lung cells, and an in vivo mammalian erythrocyte micronucleus test using Hsd:ICR (CD-1) SPF mouse bone marrow. After LK1 administration (2,500 mg/kg), there were no LK1-related body weight changes or necropsy findings. The reverse mutation test showed no increased reverse mutation upon exposure to 5,000 mu g/plate of the LK1 powder, the maximum tested amount. The chromosome aberration test and micronucleus assay demonstrated no chromosomal abnormalities and genotoxicity, respectively, in the presence of the LK1 powder. The absence of physiological findings and genetic abnormalities suggests that LK1 powder is appropriate as a candidate biomass to be used as a safe food ingredient.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38939
DOI
10.4014/jmb.2007.07013
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.31, no.2, pp.251 - 258, 2021
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
C-PHYCOCYANIN; ANTIOXIDANT; APOPTOSIS
Keywords
Leptolyngbya sp; KIOST-1; cyanobacterium; food ingredient; genetic toxicity; single-dose oral toxicity
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
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