Adaptive evolution reveals a mutation of hydrogenase effective to enhance formate-dependent hydrogen production in Thermococcus onnurineus NA1

Title
Adaptive evolution reveals a mutation of hydrogenase effective to enhance formate-dependent hydrogen production in Thermococcus onnurineus NA1
Author(s)
Jung, Hae-Chang; Lee, Seong Hyuk; Lee, Seonmin; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Lee, Seonmin(이선민)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2020-11-16
Abstract
The hyperthermophilic archaeon, Thermococcus onnurineus NA1 could grow on formate, coupled with hydrogen production. Formate conversion to hydrogen was never thought to be energetic enough to sustain cell growth. T. onnurineus NA1 was the first example that a single strain can grow on formate, producing hydrogen. The formate-dependent hydrogen production in the strain was mediated by a formate-hydrogen lyase (FHL) complex comprised of formate dehydrogenase module, hydrogenase module and Na+/H+ antiporter module. We also reported that significant changes of cell growth and hydrogen production could be observed in 156 times serially-transferred strain (WTF-156T) on formate-containing medium. While no mutation in WTF-156T was found in the genes encoding FHL complex, a mutation at formate transporter (TON_1573) caused significant increase in formate consumption and H2 production.
In this study, the WTF-156T strain was further serially-transferred over another 200 times. The resulting strain, WTF-350T strain, exhibited much faster consumption rate of formate and higher growth rate than those of WTF-156T strain. Genomic analysis of WTF-350T strain revealed additional 16 mutations on top of the 11 mutations identified in the WTF-156T strain. We focused on investigating the effect of two mutations found in formate consumption machinery: H339N mutation at the gene encoding a membrane subunit (TON_1565) of the multimeric membrane-bound hydrogenase of the FHL complex and additional mutation (S104N) at formate transporter (TON_1573). We introduced each mutation into the parent strain, and then evaluated formate consumption rate using resting cells of each mutant. The resulting mutants harboring each mutation at TON_1565 (H339N) or TON_1573 (S104N) showed higher formate consumption rate of 12% and 14% at pH 6.5 and 34% and 32% at pH 7.8, respectively, than the parent strain. The underlying mechanism to explain the change will be discussed further.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38923
Bibliographic Citation
International Union of Microbiological Societies Congresses (IUMS 2020), pp.246, 2020
Publisher
International Union of Microbiological Societies
Type
Conference
Language
English
Publisher
International Union of Microbiological Societies
Related Researcher
Research Interests

Marine extremophile,해양극한미생물

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse