A Fluorescent Probe for Selective Facile Detection of H2S in Serum Based on an Albumin-Binding Fluorophore and Effective Masking Reagent SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 6 time in Scopus
Title
A Fluorescent Probe for Selective Facile Detection of H2S in Serum Based on an Albumin-Binding Fluorophore and Effective Masking Reagent
Author(s)
Lee, Suji; Sung, Dan Bi; Lee, Jong Seok; Han, Min Su
KIOST Author(s)
Sung, Dan Bi(성단비)Lee, Jong Seok(이종석)
Alternative Author(s)
성단비; 이종석
Publication Year
2020-12-22
Abstract
A fluorescent probe (4-(2-(4-(diethylamino)-phenyl)-4-methyl-5-oxo-4,5- dihydrothieno [3,2- b]pyridin-7-yl)phenyl 2,4-dinitrobenzenesulfonate, KF-DNBS) for facile detection of H2S in serum was developed based on the combination of an environment-sensitive fluorophore (2-(4-(diethylamino)-phenyl)-7-(4-hydroxyphenyl)-4-methylthieno [3,2b] pyridin-5(4H)-one, KF) with albumin and the 2,4-dinitrobenzene sulfonyl (DNBS) group as a recognition unit for H2S. KF-DNBS showed remarkable fluorescence enhancement due to H2S-triggered thiolysis followed by the formation of a fluorescent fluorophore (KF)-albumin complex. The H2S detection limit of KF-DNBS was estimated to be 3.2 mu M, and KF-DNBS achieves a high selectivity to H2S over biothiols by employing 2-formyl benzene boronic acid (2-FBBA) as an effective masking reagent. Furthermore, under optimized sensing conditions, KF-DNBS could be applied to accurately determine spiked H2S in human serum without the need for any further procedure for the removal of serum proteins.
ISSN
2470-1343
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38913
DOI
10.1021/acsomega.0c04659
Bibliographic Citation
ACS OMEGA, v.5, no.50, pp.32507 - 32514, 2020
Publisher
AMER CHEMICAL SOC
Subject
HYDROGEN-SULFIDE; BLOOD; GASOTRANSMITTER; CYSTEINE; BIOLOGY; SULFUR; HEALTH
Type
Article
Language
English
Document Type
Article
Publisher
AMER CHEMICAL SOC
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