A Fluorescent Probe for Selective Facile Detection of H2S in Serum Based on an Albumin-Binding Fluorophore and Effective Masking Reagent
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Title
- A Fluorescent Probe for Selective Facile Detection of H2S in Serum Based on an Albumin-Binding Fluorophore and Effective Masking Reagent
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Author(s)
- Lee, Suji; Sung, Dan Bi; Lee, Jong Seok; Han, Min Su
- KIOST Author(s)
- Sung, Dan Bi(성단비); Lee, Jong Seok(이종석)
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Alternative Author(s)
- 성단비; 이종석
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Publication Year
- 2020-12-22
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Abstract
- A fluorescent probe (4-(2-(4-(diethylamino)-phenyl)-4-methyl-5-oxo-4,5- dihydrothieno [3,2- b]pyridin-7-yl)phenyl 2,4-dinitrobenzenesulfonate, KF-DNBS) for facile detection of H2S in serum was developed based on the combination of an environment-sensitive fluorophore (2-(4-(diethylamino)-phenyl)-7-(4-hydroxyphenyl)-4-methylthieno [3,2b] pyridin-5(4H)-one, KF) with albumin and the 2,4-dinitrobenzene sulfonyl (DNBS) group as a recognition unit for H2S. KF-DNBS showed remarkable fluorescence enhancement due to H2S-triggered thiolysis followed by the formation of a fluorescent fluorophore (KF)-albumin complex. The H2S detection limit of KF-DNBS was estimated to be 3.2 mu M, and KF-DNBS achieves a high selectivity to H2S over biothiols by employing 2-formyl benzene boronic acid (2-FBBA) as an effective masking reagent. Furthermore, under optimized sensing conditions, KF-DNBS could be applied to accurately determine spiked H2S in human serum without the need for any further procedure for the removal of serum proteins.
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ISSN
- 2470-1343
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38913
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DOI
- 10.1021/acsomega.0c04659
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Bibliographic Citation
- ACS OMEGA, v.5, no.50, pp.32507 - 32514, 2020
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Publisher
- AMER CHEMICAL SOC
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Subject
- HYDROGEN-SULFIDE; BLOOD; GASOTRANSMITTER; CYSTEINE; BIOLOGY; SULFUR; HEALTH
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Type
- Article
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Language
- English
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Document Type
- Article
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