Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation
SCIE
SCOPUS
Cited 4 time in
WEB OF SCIENCE
Cited 7 time in
Scopus
-
Title
- Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation
-
Author(s)
- Lee, Seong Hyuk; Lee, Sung-Mok; Lee, Jung-Hyun; Lee, Hyun Sook; Kang, Sung Gyun
- KIOST Author(s)
- Lee, Seong Hyuk(이성혁); Lee, Sung Mok(이성목); Lee, Jung Hyun(이정현); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균)
-
Alternative Author(s)
- 이성혁; 이성목; 이정현; 이현숙; 강성균
-
Publication Year
- 2020-06
-
Abstract
- To develop a thermophilic cell factory system that uses CO gas, we attempted to engineer a hyperthermophilic carboxydotrophic hydrogenic archaeon Thermococcus onnurineus NA1 to be capable of producing thermophilic enzymes along with hydrogen (H-2). The mutant strains 156T-AM and 156T-POL were constructed to have another copy of a gene encoding alpha-amylase or DNA polymerase, respectively, and exhibited growth rates and H-2 production rates distinct from those of the parental strain, 156T, in gas fermentation using 100% CO or coal-gasified syngas. Purified alpha-amylase displayed starch-hydrolyzing activity, and whole-cell extracts of 156T-AM showed saccharifying activity for potato peel waste. PCR amplification was used to demonstrate that purified DNA polymerase was free from bacterial DNA contamination, in contrast to commercial bacteria-made enzymes. This study demonstrated that this archaeal strain could coproduce enzymes and H-2 using CO-containing gas, providing a basis for cell factories to upcycle industrial waste gas.
-
ISSN
- 0960-8524
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/38643
-
DOI
- 10.1016/j.biortech.2020.123067
-
Bibliographic Citation
- BIORESOURCE TECHNOLOGY, v.305, 2020
-
Publisher
- ELSEVIER SCI LTD
-
Subject
- DEPENDENT H-2 PRODUCTION; HYPERTHERMOPHILIC ARCHAEON; DNA-POLYMERASE; ESCHERICHIA-COLI; BACTERIAL-DNA; SYNTHESIS GAS; POTATO PEEL; SEQUENCE; DENSITY; CLONING
-
Keywords
- Cell factory; Thermococcus onnurineus NA1; Coproduction; Hydrogen; Thermophilic enzyme
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.